乙醇灌胃对兔股骨头内骨内压和骨小梁结构的影响

发布时间：2018-04-13 20:53

  本文选题：酒精性股骨头坏死 + 骨内压　； 参考：《山东医药》2017年48期

【摘要】：目的探讨乙醇灌胃对兔股骨头内骨内压及骨小梁结构的影响。方法将48只成年雄性新西兰大白兔随机分为观察组和对照组,每组24只。观察组给予42%乙醇灌胃,对照组给予生理盐水灌胃,剂量均为10 m L/(kg·d),3次/周,连续灌胃6个月。分别于灌胃前及灌胃1、3、6个月时检测两组左后肢股骨头内骨内压;处死家兔,取其右后肢股骨头组织,石蜡包埋,4μm厚切片,HE染色,显微镜下观察,计算骨小梁面积分数及空骨陷窝率。结果两组干预前及灌胃1个月时,股骨头内骨内压、骨小梁面积分数及空骨陷窝率比较差异均无统计学意义(P均0.05);灌胃3、6个月时,观察组股骨头内骨内压、空骨陷窝率均高于对照组,骨小梁面积分数均低于对照组,组间比较P0.05或0.01。结论长时间(≥3个月)乙醇灌胃可使兔股骨头内骨内压升高,骨小梁结构改变。
[Abstract]:Objective to investigate the effect of ethanol on intraosseous pressure and trabecular structure of femoral head in rabbits.Methods 48 adult male New Zealand white rabbits were randomly divided into observation group and control group with 24 rabbits in each group.The observation group was given 42% ethanol and the control group was given normal saline for 3 times a week for 6 months.The intraosseous pressure of the femoral head of the left hind limb of the two groups was measured before gastric perfusion and at the end of 6 months, and the femoral head of the right hind limb of the rabbits were killed, and the tissues of the femoral head of the right hind limb were taken out, and the paraffin embedded 4 渭 m thick sections were stained with HE, and observed under microscope.The trabecular area fraction and the rate of empty bone lacuna were calculated.Results there were no significant differences in intraosseous pressure, trabecular area fraction and empty bone lacuna rate between the two groups before and after 1 month of gastric perfusion (P < 0.05), but there was no significant difference in intraosseous pressure of femoral head between the two groups at 3 and 6 months after gastric perfusion.The rate of empty bone lacuna was higher than that of control group, and the area fraction of trabecular bone was lower than that of control group (P0.05 or 0.01).Conclusion ethanol administration for a long time (鈮，

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