PRP明胶海绵复合物在前交叉韧带重建术后腱骨愈合的作用

发布时间：2018-04-14 12:14

本文选题：富血小板血浆 + 明胶海绵　；参考：《中国矫形外科杂志》2017年08期

【摘要】：[目的]观察复合有富血小板血浆(platlet rich plasma,PRP)明胶海绵复合物的自体肌腱移植物对前交叉韧带重建术后骨隧道与肌腱间愈合过程的影响,探讨其在腱与骨愈合中发挥的效果。[方法]新西兰大白兔36只,无菌条件下获取自体PRP,制备PRP明胶海绵复合物,左下肢行自体跟腱移植前交叉韧带重建术。A组(实验组)使用PRP明胶海绵复合物;B组(对照组1)使用PRP;C组(对照组2)仅为肌腱。植入2、4、8、12周后,制作标本,进行大体及HE、Masson染色和TGF-β1免疫组化观察。[结果]A组标本大体观察:前交叉韧带连续性及张力良好,术后2周骨隧道口见少量瘢痕组织;4周骨道口见小部分骨样组织,8周骨道口较多瘢痕呈现,12周骨道口现较多骨样成分。组织学观察:HE染色显示,A组出现Sharpey样纤维和软骨细胞明显早于B组和C组;同样在Masson结果中,A组出现有规则的胶原纤维也明显多于B组和C组。TGF-β1免疫组织化学观察:2、4、8周,A组阳性率明显高于B、C组,差异具有统计学意义(P0.05)。而在术后12周时,各组之间的表达无明显差异(P0.05)。[结论]PRP明胶海绵复合物可促进前交叉韧带重建后腱骨的早期愈合。
[Abstract]:[objective] to observe the effect of autologous tendon grafts combined with platlet rich plasma-PRP sponge complex on the healing process between bone tunnel and tendon after anterior cruciate ligament reconstruction, and to explore its effect on tendon and bone union.[methods] PRP gelfoam complex was prepared from 36 New Zealand white rabbits.Anterior cruciate ligament reconstruction with autologous Achilles tendon transplantation in left lower extremity. Group A (experimental group) was treated with PRP gelfoam complex B (control group 1) and group C (control group 2) was only tendon.After 12 weeks of implantation, the specimens were made, and the gross and HE Masson staining and TGF- 尾 1 immunohistochemistry were observed.[results] in group A, there was good continuity and tension of anterior cruciate ligament.At 2 weeks after operation, a small amount of scar tissue was found at the entrance of bone tunnel. At 4 weeks after operation, a small part of bone tissue was found in the orifice of bone. More scars appeared in the orifice of bone at 8 weeks after operation, and there were more bone like components in the orifice of bone at 12 weeks after operation.Histological observation showed that Sharpey like fibers and chondrocytes appeared earlier in group A than those in group B and group C.In the same Masson results, the number of regular collagen fibers in group A was significantly higher than that in group B and group C, and the positive rate in group A was significantly higher than that in group B and group C (P 0.05), and the positive rate in group A was significantly higher than that in group B (P 0.05).At 12 weeks after operation, there was no significant difference in the expression of P0. 05.[conclusion] PRP gelfoam complex can promote the early healing of tendon bone after anterior cruciate ligament reconstruction.
【作者单位】：西安交通大学医学院附属红会医院运动医学科;
【基金】：陕西省自然科学基金项目(编号:2016JM8138) 陕西省卫计委科研基金项目(编号:2014D51)
【分类号】：R687.4

【相似文献】