七氟烷复合咪达唑仑麻醉对老年大鼠认知功能的影响及其机制

发布时间：2018-04-17 11:23

本文选题：七氟烷 + 咪达唑仑　；参考：《中国老年学杂志》2017年08期

【摘要】：目的探讨七氟烷复合咪达唑仑麻醉对老年大鼠认知功能的影响及其机制。方法将64只SD大鼠随机分为对照组(C组)、七氟烷组(S组)、咪达唑仑组(M组)、七氟烷复合咪达唑仑组(S+M组),每组大鼠16只,处理后采用Morris水迷宫实验检测各组大鼠认知功能的变化以及各组大鼠海马N-甲基-D-天冬氨酸受体(NR)1、NR2B mRNA表达情况、各组大鼠海马神经型一氧化氮合酶(n NOS)、半胱氨酸蛋白酶(caspase)-3蛋白表达。结果 Morris水迷宫实验训练1 d,各组大鼠潜伏期比较差异无统计学意义(P0.05),训练3 d、5 d,各麻醉组大鼠潜伏期均显著高于C组,差异有统计学意义(P0.05),且S+M组大鼠潜伏期明显高于S组及M组(P0.05),差异有统计学意义;将平台撤除后,各麻醉组大鼠60 s内穿过平台次数明显低于C组,差异有统计学意义(P0.05),且S+M组大鼠穿过平台次数明显低于S组及M组,差异有统计学意义(P0.05);各组大鼠海马NR1 mRNA相对表达量比较差异无统计学意义(P0.05),S组与S+M组大鼠海马NR2B mRNA相对表达量较C组、M组显著降低,差异有统计学意义(P0.05),M组与C组大鼠海马NR2B mRNA相对表达量差异无统计学意义(P0.05);与C组比较,各麻醉组大鼠海马n NOS蛋白表达降低,差异有统计学意义,且S+M组大鼠海马n NOS蛋白表达量最低,而各组大鼠海马caspase-3蛋白表达情况无明显变化,差异有统计学意义(P0.05)。结论七氟烷复合咪达唑仑可引起大鼠认知功能的降低,其机制可能与抑制NR2B、NOS表达有关,而与神经细胞凋亡无明显关系。
[Abstract]:Objective to investigate the effect and mechanism of sevoflurane combined with midazolam anesthesia on cognitive function in aged rats.Methods Sixty-four SD rats were randomly divided into control group (C), sevoflurane group (group S), midazolam group (group M), sevoflurane combined with midazolam group (group S M), and sevoflurane combined with midazolam group (n = 16).After treatment, the changes of cognitive function and the expression of N- methyl-Daspartic acid receptor (NR1) NR2B mRNA in hippocampus of rats in each group were detected by Morris water maze test.The expression of neuronal nitric oxide synthase (nNOS) and cysteine protease (Caspase-3) in hippocampus of rats in each group.Results Morris water maze experiment training for 1 day, there was no significant difference in the latency of each group (P 0.05), training 3 days and 5 days, the latency of each anesthetic group was significantly higher than that of C group.The latency of rats in S M group was significantly higher than that in S group and M group (P 0.05).The difference was statistically significant (P 0.05), and the times of crossing the platform in S M group were significantly lower than those in S group and M group.There was no significant difference in the relative expression of NR1 mRNA between the two groups. The relative expression of NR2B mRNA in the hippocampus of S group and S M group was significantly lower than that of C group.There was no significant difference in the relative expression of NR2B mRNA between group M and group C (P 0.05), but the expression of n NOS protein in hippocampus of all anesthetized groups was lower than that of group C (P 0.05).The expression of n NOS protein in hippocampus of S M group was the lowest, but the expression of caspase-3 protein in hippocampus of all groups had no significant change (P 0.05).Conclusion sevoflurane combined with midazolam can induce the decrease of cognitive function in rats, and its mechanism may be related to the inhibition of NOS expression in NR2BNs, but not to neuronal apoptosis.
【作者单位】：郑州大学附属肿瘤医院麻醉科;郑州大学医学院;
【基金】：河南省医学科技攻关计划(No.122300410408)
【分类号】：R614

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