鸡胚尿囊膜载羟基磷灰石血管化模型的建立

发布时间：2018-04-18 20:27

本文选题：羟基磷灰石类 + 新生血管化　；参考：《中国组织工程研究》2017年26期

【摘要】：背景:利用第8-12天尿囊膜血管的快速生长期,可模拟骨缺损患者骨断端血管生长和植骨代替材料进入人体后的血管植入过程。目的:将羟基磷灰石负载于鸡胚尿囊膜模型,建立植入体血管化的研究模型。方法:将12枚罗曼品系鸡蛋于相同适宜条件下孵化至第8天,开窗后随机分为2组,实验组植入羟基磷灰石材料,建立鸡胚尿囊膜载羟基磷灰石模型;对照组放入滤纸材料,密封后移入恒温恒湿箱内继续孵化。待孵化至第12天后,观察两组标本尿囊膜4级以上血管生成长度及单位面积血管生成长度,以及羟基磷灰石及滤纸材料上的血管新生状况。结果与结论:(1)待孵化12 d后,实验组标本平均尿囊膜血管新生成长度为24.031 mm,平均单位面积血管新生成长度为0.242 mm/mm2;对照组标本平均尿囊膜血管新生成长度为23.561 mm,平均单位面积血管新生成长度为0.212 mm/mm2,组间血管新生成长度及平均尿囊膜血管新生成长度比较差异无显著性意义;(2)实验组羟基磷灰石材料切片标本有明显类血管组织;(3)结果表明,鸡胚尿囊膜载羟基磷灰石模型的建立对理想植骨材料及改变血管生成药物的研究提供了简单准确且高效的体外动物模型,且外载羟基磷灰石对尿囊膜上血管生成无影响。
[Abstract]:Background: by using the rapid growth phase of allantoic membrane vessels on the 8th to 12th day, the vascular growth at the broken end of bone and the implantation of bone graft into human body can be simulated.Aim: to establish a vascularization model of chicken embryo with hydroxyapatite loaded on allantoic membrane.Methods: twelve Roman eggs were incubated under the same suitable conditions until the 8th day. After opening the window, 12 eggs were randomly divided into two groups: the experimental group was implanted with hydroxyapatite material, the chicken embryo allantoic membrane loaded hydroxyapatite model was established, and the control group was added filter paper material.After sealing, move into the constant temperature and humidity box to continue hatching.After hatching to the 12th day, the angiogenesis length and angiogenesis length per unit area of the allantoic membrane above grade 4 were observed, and the angiogenesis status of hydroxyapatite and filter paper materials were also observed.Results and conclusion (1) after incubation for 12 days,The average new angiogenesis length of allantoic membrane was 24.031 mm and the average angiogenesis length per unit area was 0.242 mm / mm 2 in the experimental group and 23.561 mm in the control group, and the average new angiogenesis length per unit area was 23.561 mm in the control group.There was no significant difference in angiogenesis and the average angiogenesis length of the allantoic membrane between the two groups (0.212 mm / m2).The establishment of chicken embryo allantoic membrane loaded hydroxyapatite model provided a simple, accurate and efficient animal model for the study of ideal bone graft materials and angiogenesis drugs, and the exogenous hydroxyapatite had no effect on the angiogenesis of the allantoic membrane.
【作者单位】：西南医科大学;解放军成都军区总医院附属口腔医院;
【分类号】：R318.08;R68

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