盐酸戊乙奎醚对新生大鼠内毒素性急性肺损伤时肺氧化应激和细胞凋亡的影响

发布时间：2018-04-18 22:14

本文选题：胆碱能拮抗剂 + 细胞凋亡　；参考：《临床麻醉学杂志》2017年07期

【摘要】：目的评价盐酸戊乙奎醚对新生大鼠内毒素性急性肺损伤(acute lung injury,ALI)时肺氧化应激和细胞凋亡的影响。方法清洁级健康雄性Wistar大鼠30只,7日龄,体重12~18g。采用随机数字表法分为三组:盐酸戊乙奎醚组(PHC组)、ALI组和生理盐水组(NS组),每组10只。PHC组和ALI组大鼠腹腔注射内毒素5.0mg/kg制备ALI模型。PHC组于内毒素注射前1h腹腔注射盐酸戊乙奎醚2.0mg/kg,NS组和ALI组给予等容量生理盐水。于注射内毒素4h后处死大鼠取肺组织标本,计算肺湿/干重比(W/D),采用硫代巴比妥酸法测定丙二醛(MDA)浓度,黄嘌呤氧化酶法测定大鼠超氧化物歧化酶(SOD)活性,免疫组织化学法测定细胞色素C(Cyt-C)、半胱氨酸天冬蛋白酶-3(Caspase-3)的含量,TUNEL法计数凋亡细胞,计算细胞凋亡指数(AI)。结果与NS组比较,ALI组和PHC组肺W/D和MDA浓度明显升高,SOD活性明显降低(P0.05);与ALI组比较,PHC组肺W/D和MDA浓度明显降低,SOD活性明显升高(P0.05)。与NS组比较,ALI组和PHC组Cyt-C、Caspase-3含量和AI明显升高;与ALI组比较,PHC组Cyt-C、Caspase-3含量和AI明显降低(P0.05)。结论盐酸戊乙奎醚可能通过抑制肺组织氧化应激和细胞凋亡,减轻新生大鼠内毒素性急性肺损伤。
[Abstract]:Objective to evaluate the effects of pentehyclidine hydrochloride on lung oxidative stress and apoptosis in neonatal rats with endotoxin-induced acute lung injury (Ali).Methods 30 healthy male Wistar rats of clean grade were aged 7 days, weighing 12 ~ 18g.Three groups were randomly divided into three groups: penehyclidine hydrochloride group (PHC group) and normal saline group (NS group). 10 rats in each group and ALI group were injected endotoxin 5.0mg/kg intraperitoneally to make ALI model. PHC group was injected intraperitoneally 1 hour before endotoxin injection.Isovolumic NS and ALI groups were given isovolumic saline.Four hours after endotoxin injection, rats were killed to collect lung tissue samples, and lung wet / dry weight ratio was calculated. The concentration of malondialdehyde (MDA) was measured by thiobarbituric acid method, and the activity of superoxide dismutase (SOD) was determined by xanthine oxidase method.The contents of cytochrome Cyt-Con and cysteine aspartate protease-3 (Caspase-3) were determined by immunohistochemical method and the apoptotic cells were counted by Tunel method and the apoptotic index was calculated.Results compared with NS group, lung WR / D and MDA concentration in Ali group and PHC group were significantly increased, sod activity was decreased significantly, and lung WR / D and MDA concentration in ALI group was significantly decreased compared with that in ALI group.Compared with NS group, the content and AI of Cyt-Cncaspase-3 in Ali group and PHC group were significantly increased, and the content and AI of Caspase-3 in ALI group were significantly lower than those in ALI group.Conclusion Penehyclidine hydrochloride may reduce endotoxin induced acute lung injury by inhibiting oxidative stress and apoptosis of lung tissue in neonatal rats.
【作者单位】：郑州大学附属肿瘤医院麻醉科;郑州大学第三附属医院麻醉科;
【分类号】：R614

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