国产牛心包补片在羊肺动脉和主动脉补片实验中的安全性和有效性

发布时间：2018-04-20 00:36

本文选题：牛心包补片 + 国产补片　；参考：《复旦学报(医学版)》2017年02期

【摘要】：目的本研究对国产牛心包补片的安全性和有效性进行评价,为进一步临床应用提供实验基础。方法采用绵羊肺动脉及降主动脉补片植入模型,以4只羊作为实验组,在肺动脉干和降主动脉干两处共植入8枚国产牛心包补片;以2只羊作为对照组,在肺动脉干和降主动脉干两处共植入4枚进口牛心包补片。术前对所有动物进行常规生化检查,术后第1个月、第2个月随访超声心动图,第3个月时进行取材,比较两组植入补片的内皮化、炎症和钙化程度等。结果 (1)所有动物均存活,未发生手术相关并发症;(2)超声心动图第1个月、第2个月的结果显示肺动脉和主动脉补片内无缝合处漏血、无钙化、无血栓形成、无赘生物、无明显动脉瘤样扩张;(3)肉眼观察两组补片均无内膜增生,厚度和韧度较术前无明显改变;两组补片内膜光滑,均无肉眼可见的钙化和异物。(4)微生物学检测提示两组补片均未出现微生物感染,均有良好的内皮化,均有炎症细胞浸润,坏死、钙化程度差异无显著统计学意义。结论国产牛心包补片在绵羊肺动脉和主动脉补片试验中具有良好的安全性和有效性。
[Abstract]:Objective to evaluate the safety and efficacy of domestic bovine pericardial patch in order to provide experimental basis for further clinical application. Methods using sheep pulmonary artery and descending aorta patch implantation model, 4 sheep as experimental group, a total of 8 domestic cattle pericardial patch were implanted in pulmonary artery trunk and descending aortic trunk, and 2 sheep were used as control group. Four imported bovine pericardial patches were implanted in the pulmonary trunk and descending aortic trunk. All animals were examined by routine biochemical examination before operation. Echocardiography was followed up in the first month and the second month after operation. The endothelium inflammation and calcification were compared between the two groups at the third month. Results 1) all the animals survived, and no operative complications occurred. The results of echocardiography at the first month and the second month showed that there was no blood leakage, no calcification, no thrombus, no neoplasm in pulmonary artery and aorta patch. There was no intimal hyperplasia, thickness and toughness of the two groups were not obviously changed compared with those before operation, and the intima of the two groups were smooth and smooth. There was no visible calcification and foreign body. 4) Microbiological examination showed that there was no microbe infection in both groups, and there was no significant difference in inflammatory cell infiltration, necrosis and calcification between the two groups. Conclusion domestic bovine pericardium patch has good safety and efficacy in sheep pulmonary artery and aortic patch test.
【作者单位】：复旦大学附属中山医院心外科;
【基金】：国家自然科学基金(81501595)~~
【分类号】：R654.2

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