对顺铂耐药的骨肉瘤细胞干细胞特性变化及其与Notch信号通路的关系

发布时间：2018-04-20 17:38

本文选题：骨肉瘤 + 顺铂耐药　；参考：《山东医药》2016年48期

【摘要】：目的探讨对顺铂耐药的骨肉瘤细胞干细胞特性变化及其与Notch信号通路的关系。方法取对数生长期的对顺铂耐药的人骨肉瘤143B-TK细胞作为观察组,以未经顺铂作用的人骨肉瘤143B-TK细胞作为对照组。采用实时荧光定量PCR法检测两组干细胞相关基因(TERT、Sox2、Oct4、NANOG)、Notch信号通路相关基因(Hey L、Hey2、Hey1、Hes5、Hes1)mRNA相对表达量;两组加入或不加入Notch信号通路特异性抑制剂DAPT,采用悬浮克隆球试验检测成球能力(以悬浮克隆球数量表示)。结果两组NANOG、Hey2 mRNA相对表达量比较差异均无统计学意义(P均0.05)。观察组TERT、Sox2、Oct4、Hey L、Hey1、Hes5、Hes1 mRNA相对表达量均高于对照组(P0.05或0.01)。不加入DAPT的观察组悬浮克隆球数量明显多于加入DAPT的观察组和不加入DAPT的对照组(P均0.01)。结论对顺铂耐药的骨肉瘤细胞干细胞特性增强;Notch信号通路激活可能是导致其干细胞特性增强和细胞耐药的主要原因。
[Abstract]:Objective to investigate the change of stem cell characteristics of cisplatin-resistant osteosarcoma cells and its relationship with Notch signaling pathway. Methods 143B-TK cells of cisplatin resistant human osteosarcoma at logarithmic growth stage were used as observation group and 143B-TK cells without cisplatin as control group. The relative expression of Hes5Hes1 mRNA in two groups of stem cell related genes, namely, Hes5Hes1 and Hes5Hes1, was detected by real-time fluorescence quantitative PCR assay. In two groups, Notch signaling pathway specific inhibitor DAPT was added or not, and the ability of spheroidization was tested by suspension clone ball test (expressed by the number of suspension clone balls). Results there was no significant difference in the relative expression of NANOGN Hey2 mRNA between the two groups (P < 0.05). The relative expression of hes5Hes1 mRNA in the observation group was higher than that in the control group (P0.05 or 0.01). The number of suspension clones in the observation group without DAPT was significantly higher than that in the observation group without DAPT and the control group without DAPT (P < 0.01). Conclusion the enhancement of stem cell characteristics of cisplatin resistant osteosarcoma cells and the activation of Notch signaling pathway may be the main reasons for the enhancement of stem cell characteristics and cell resistance of cisplatin resistant osteosarcoma cells.
【作者单位】：武汉大学人民医院;
【基金】：国家自然科学基金资助项目(81341078)
【分类号】：R738.1

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