植入含感觉神经束和血管束组织工程骨修复股骨大段骨缺损:降钙素基因相关肽Ⅰ型受体的中长期表达

发布时间：2018-04-21 05:17

  本文选题：神经节 + 感觉　； 参考：《中国组织工程研究》2017年06期

【摘要】：背景:前期实验证实,植入含血管束或感觉神经的组织工程骨促成骨程度与其内的感觉神经肽受体表达相关。目的:观察植入含感觉神经束和血管束的组织工程骨修复兔股骨大段骨缺损,对降钙素基因相关肽Ⅰ型受体中长期表达的影响。方法:取36只新西兰大白兔,制作单侧股骨1.5 cm缺损模型,随机分3组,感觉神经组于骨缺损处植入成骨诱导7 d的骨髓间充质干细胞-β-磷酸三钙组织工程骨,并在组织工程骨侧槽内植入自体感觉神经束;血管束组于骨缺损处植入成骨诱导7 d的骨髓间充质干细胞-β-磷酸三钙组织工程骨,并在组织工程骨侧槽内植入自体股血管束;空白对照组于骨缺损处植入成骨诱导7 d的骨髓间充质干细胞-β-磷酸三钙组织工程骨。植入后24,48周,进行X射线、免疫组织学及荧光定量PCR检测。结果与结论:1X射线检查:感觉神经组、血管束组X射线评分高于空白对照组(P0.05),感觉神经组、血管束组X射线评分比较差异无显著性意义;2荧光定量PCR检测:血管束组降钙素基因相关肽Ⅰ型受体表达量高于感觉神经组、空白对照组(P0.05),感觉神经组与空白对照组比较差异无显著性意义;3免疫组织化学观察:感觉神经组、血管束组降钙素基因相关肽Ⅰ型受体阳性表达强于空白对照组;4结果表明:植入含血管束的组织工程骨可促进降钙素基因相关肽Ⅰ型受体的表达。
[Abstract]:Background: previous studies have demonstrated that the degree of bone promotion by implanting tissue engineered bone containing vascular bundles or sensory nerves is related to the expression of sensory neuropeptide receptors. Aim: to investigate the effect of implanted tissue engineered bone containing sensory nerve bundle and vascular bundle on the long-term expression of calcitonin gene-related peptide type I receptor in rabbit femur. Methods: 36 New Zealand white rabbits were randomly divided into 3 groups. The sensory nerve group implanted bone marrow mesenchymal stem cells (BMSCs)-尾 -tricalcium phosphate tissue engineering bone at the site of bone defect for 7 days. Autogenous sensory nerve bundle was implanted into the lateral groove of tissue engineering bone, and bone marrow mesenchymal stem cell-尾-tricalcium phosphate tissue engineering bone was implanted in the blood tube bundle group at the site of bone defect for 7 days, and autogenous femoral vascular bundle was implanted in the tissue engineering bone side groove. Bone marrow mesenchymal stem cells (BMSCs)-尾-tricalcium phosphate tissue engineering bone was implanted at bone defect in blank control group. X-ray, immunohistochemistry and fluorescence quantitative PCR were performed 48 weeks after implantation. Results and conclusion: the X-ray score of sensory nerve group and blood bundle group was higher than that of blank control group (P 0.05), sensory nerve group (P 0.05), sensory nerve group (P < 0.05), and sensory nerve group (P < 0.05). There was no significant difference in X-ray score between vascular bundle group and vascular bundle group. The expression of calcitonin gene-related peptide type I receptor in vascular bundle group was higher than that in sensory nerve group, and the expression of calcitonin gene-related peptide type 鈪，

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