促红细胞生成素对地塞米松冲击大鼠股骨头坏死的预防作用及机制

发布时间：2018-04-24 08:04

本文选题：促红细胞生成素 + 地塞米松　；参考：《中国老年学杂志》2017年04期

【摘要】：目的探讨促红细胞生成素对地塞米松冲击大鼠股骨头坏死的预防作用及机制。方法 SD雄性大鼠36只随机分为正常组、地塞米松组和实验组,每组12只。地塞米松组和实验组大鼠尾静脉注射脂多糖20μg/kg,连续2 d,第2次给予脂多糖注射后,当日肌肉注射地塞米松20 mg/kg,连续注射3 d。结果与正常组比较,地塞米松组和实验组全血低切、全血高切、血细胞比容水平变化均上升,且实验组均明显低于地塞米松组(P0.05);与正常组比较,地塞米松组血钙(Ca)、血磷(P)、骨密度明显下降(P0.05),而实验组均无明显变化(P0.05);实验组血钙、血磷、骨密度明显高于地塞米松组(P0.05);与正常组比较,地塞米松组和实验组血浆一氧化氮(NO)水平下降,内皮素(ET)水平上升(P0.05);实验组血浆NO水平高于地塞米松组,ET水平低于地塞米松组(P0.05);正常组大鼠骨小梁完整及股骨头软骨完整;地塞米松组大鼠骨小梁有坏死现象,且股骨头软骨下陷,造血细胞数量降低;实验组大鼠较地塞米松组明显改善。结论促红细胞生成素对地塞米松冲击大鼠股骨头坏死具有良好的预防作用,其作用机制可能与改善血液流变学,提高血磷、血钙、骨密度及改善血管内皮功能紊乱相关。
[Abstract]:Objective to investigate the preventive effect and mechanism of erythropoietin (EPO) on osteonecrosis of femoral head in rats with dexamethasone impact. Methods Thirty-six SD male rats were randomly divided into normal group, dexamethasone group and experimental group with 12 rats in each group. In the dexamethasone group and experimental group, 20 渭 g / kg lipopolysaccharide (LPS) was injected into the tail vein of rats for 2 days. After the second injection of LPS, dexamethasone was injected intramuscularly at 20 mg / kg for 3 days. Results compared with the normal group, the dexamethasone group and the experimental group had lower whole blood cut, higher whole blood shear, higher blood volume, and lower blood volume than that of the dexamethasone group (P 0.05), and compared with the normal group, the blood volume in the experimental group was significantly lower than that in the control group. In the dexamethasone group, the bone mineral density (BMD) decreased significantly (P 0.05), but there was no significant change in the experimental group (P 0.05), and the blood calcium, phosphorus and bone mineral density (BMD) in the experimental group were significantly higher than those in the dexamethasone group (P 0.05). The plasma nitric oxide (no) level in dexamethasone group and experimental group decreased, the endothelin (et) level increased (P 0.05), the plasma no level in experimental group was higher than that in dexamethasone group (P 0.05), the bone trabeculae integrity and femoral head cartilage integrity in normal group were lower than that in dexamethasone group (P 0.05). In the dexamethasone group, the trabecular bone necrosis was observed, and the femoral head cartilage fell down, and the number of hematopoietic cells decreased, while the experimental group was significantly improved compared with the dexamethasone group. Conclusion Erythropoietin has a good preventive effect on femoral head necrosis induced by dexamethasone in rats, and its mechanism may be related to improving hemorheology, increasing blood phosphorus, blood calcium, bone mineral density and improving vascular endothelial dysfunction.
【作者单位】：吉林省武警总队医院骨科;
【分类号】：R681.8

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