塞来昔布对人成骨细胞增殖的影响及其作用机制

发布时间：2018-04-24 18:49

本文选题：环氧化酶- + 塞来昔布　；参考：《安徽医科大学学报》2015年05期

【摘要】：目的观察塞来昔布对人成骨细胞增殖的影响及其作用机制。方法将人成骨细胞分为3个组:空白对照组、IL-1刺激组(阴性对照组)、塞来昔布+IL-1刺激组(药物干预组),用RT-PCR法和Western blot法分别检测三组成骨细胞环氧化酶-2(COX-2)、膜相关前列腺素E2合成酶1(m PGES-1)mRNA和蛋白的表达。MTT法检测塞来昔布对阴性对照组人成骨细胞增殖的影响。结果 RT-PCR法和Western blot法实验结果显示,同空白对照组相比,阴性对照组的人成骨细胞中COX-2、m PGES-1 mRNA和蛋白的表达均增加;同阴性对照组相比,药物干预组的COX-2、m PGES-1mRNA和蛋白的表达均降低。塞来昔布对IL-1刺激的人成骨细胞的生长具有抑制作用,呈现剂量依赖性,浓度越大,抑制作用越大;并且随着作用时间的延长,细胞的抑制作用增大。结论塞来昔布可以抑制炎性状态下人成骨细胞的生长,可能是通过调控COX-2、m PGES-1 mRNA和蛋白的表达,使其表达下调而发挥作用。
[Abstract]:Objective to observe the effect of celecoxib on the proliferation of human osteoblasts and its mechanism. Methods Human osteoblasts were divided into three groups: blank control group (negative control group) and celecoxib IL-1 stimulation group (Celecoxib IL-1 stimulation group). The membrane phase of osteoblasts was detected by RT-PCR method and Western blot method. The effect of celecoxib on the proliferation of human osteoblasts in negative control group was detected by MTT assay. Results the results of RT-PCR assay and Western blot assay showed that the expression of COX-2 m PGES-1 mRNA and protein in osteoblasts in negative control group was higher than that in blank control group, but the expression of COX-2 m PGES-1mRNA and protein in drug intervention group was lower than that in negative control group. Celecoxib inhibited the growth of human osteoblasts stimulated by IL-1 in a dose-dependent manner. Conclusion Celecoxib can inhibit the growth of human osteoblasts in inflammatory state, which may play a role by regulating the expression of COX-2 PGES-1 mRNA and protein and down-regulating the expression of celecoxib.
【作者单位】：安徽医科大学第一附属医院骨科;
【基金】：安徽省自然科学基金(编号:1408085MH182)
【分类号】：R681

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