BMP-2和IGF-1基因治疗糖尿病骨折延迟愈合的作用和机制

发布时间：2018-04-25 18:17

  本文选题：糖尿病 + 骨折延迟愈合　； 参考：《中国老年学杂志》2017年15期

【摘要】：目的探讨骨形态发生蛋白(BMP)-2、胰岛素生长因子(IGF)-1基因治疗糖尿病(DM)骨折延迟愈合的作用和机制。方法选取6~7周龄雄性Wistar大鼠150只,分为骨折对照组、DM骨折组和治疗组。利用酶联免疫试剂盒检测各组大鼠血清中BMP-2、IGF-1的表达;测定各组大鼠血清中碱性磷酸酶(ALP)的含量,以判断对成骨细胞生长的影响;利用放射免疫竞争法检测血清中骨钙素的含量变化;利用局部骨密度仪检测骨折区域局部骨密度的变化情况。结果术后前3 w,DM骨折组与骨折对照组BMP-2、IGF-1的浓度均呈上升趋势,第3周达到最大值,治疗组BMP-2含量在第2周达到最大值,IGF-1的浓度在第3周达到最大,随后开始下降、实验中,治疗组BMP-2、IGF-1的表达量最高,DM骨折组表达量始终最低。DM骨折组ALP与骨钙素的含量始终显著低于骨折对照组与治疗组,骨折前3 w,治疗组ALP与骨钙素的含量显著低于对照组骨折无显著差异(P0.05)。骨折区域局部骨密度检测结果显示治疗组骨密度最高,骨折对照组其次,DM骨折组骨密度最低。结论 BMP-2、IGF-1基因通过提高DM大鼠骨折愈合过程中与成骨细胞增殖成熟相关的细胞因子的表达,促进骨折愈合过程中骨折区域局部骨密度的增加,从而显著改善DM骨折延迟愈合情况。
[Abstract]:Objective to investigate the effect and mechanism of bone morphogenetic protein BMP-2, IGF-1 gene on delayed healing of DM fracture. Methods 150 male Wistar rats aged 6 weeks and 7 weeks were divided into two groups: fracture group and treatment group. The expression of BMP-2TIGF-1 in serum and the content of alkaline phosphatase (ALP) in serum of rats in each group were detected by enzyme-linked immunosorbent assay (Elisa) to determine the effect on the growth of osteoblasts. The changes of serum osteocalcin and bone mineral density in fracture area were detected by radioimmunoassay. Results the concentration of BMP-2 IGF-1 in the DM fracture group and the fracture control group increased in the first 3 weeks after operation and reached the maximum at the third week. The BMP-2 content in the treatment group reached the maximum at the second week and then began to decrease. The expression of BMP-2 and IGF-1 was the highest in the treatment group and the lowest in the DM fracture group. The contents of ALP and osteocalcin in the DM fracture group were significantly lower than those in the fracture control group and the treatment group. The levels of ALP and osteocalcin in the treatment group were significantly lower than those in the control group 3 weeks before fracture (P 0.05). The local bone mineral density in fracture area was the highest in the treatment group and the lowest in the DM fracture group in the fracture control group. Conclusion BMP-2 + IGF-1 gene enhances the expression of cytokines related to the proliferation and maturation of osteoblasts in the process of fracture healing in DM rats, and promotes the increase of bone mineral density in the fracture region during fracture healing. The delayed union of DM fracture was improved significantly.
【作者单位】： 杭州市解放军117医院骨科;安徽医科大学解放军九八临床学院;
【基金】：浙江省自然科学基金资助项目(No.Y2080991)
【分类号】：R587.2;R683
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本文编号：1802420