兔自体富血小板血浆对兔ACL重建后腱骨愈合的影响

发布时间：2018-04-26 04:23

本文选题：前交叉韧带 + 富血小板血浆　；参考：《安徽医科大学学报》2016年09期

【摘要】：目的探讨兔自体富血小板血浆(PRP)对前交叉韧带(ACL)重建后腱骨愈合的作用。方法选取30只新西兰大白兔,兔龄体重相仿,随机均分为实验组和对照组。实验组每只抽取自体全血,二次离心法获得PRP。实验组移植入PRP和生物蛋白凝胶复合物;对照组移植入生物蛋白凝胶。分别于4、8、12周3个时间点处死5只兔子取材,3只用于骨道内移植物抗拉力试验,采用独立样本t检验比较;2只用于观察形态学,对上述3个时间点的两组标本分别行腱骨界面Buark评分,采用Wilcoxon秩和检验,观察ACL重建术后腱骨愈合的情况。结果 Masson三色染色显示实验组术后8周前胶原纤维增生不明显,排列混乱,8周后开始到12周胶原纤维开始大量增生,排列逐渐有序;而对照组8周及12周只见到少量排列无序的胶原纤维。移植物抗牵拉实验显示实验组和对照组的股骨道移植物抗牵拉强度与时间长度呈正比,由8周开始PRP组明显强于对照组(P0.01)。结论PRP对腱骨界面胶原纤维形成以及生物力学强度有促进作用。
[Abstract]:Objective to investigate the effect of autologous platelet-rich plasma proprp (PRP) on tendon bone healing after anterior cruciate ligament (ACLL) reconstruction. Methods 30 New Zealand white rabbits were randomly divided into experimental group and control group. In the experimental group, the whole blood was extracted and the PRP was obtained by secondary centrifugation. The experimental group was transplanted into PRP and bioprotein gel complex, and the control group was transplanted into bioprotein gel. At 12 weeks, 5 rabbits were killed at 3 time points, 3 rabbits were used for bone graft tensile test, and 2 rabbits were used to observe morphology by t test. The tendon bone interface (Buark) score was performed in the two groups at the above three time points. The healing of tendon bone after ACL reconstruction was observed by Wilcoxon rank sum test. Results Masson trichrome staining showed that the proliferation of collagen fibers was not obvious 8 weeks after operation in the experimental group, and a large number of collagen fibers began to proliferate from 8 weeks to 12 weeks after operation, and the arrangement was gradually orderly. In the control group, only a few disordered collagenous fibers were observed at 8 and 12 weeks. The tensile strength of femur grafts in the experimental group and the control group was in direct proportion to the length of time, and the PRP group was significantly stronger than the control group (P 0.01) since 8 weeks. Conclusion PRP can promote the formation of collagen fibers and biomechanical strength at the interface of tendon and bone.
【作者单位】：安徽医科大学第一附属医院运动创伤与关节镜外科;
【基金】：安徽省自然科学基金(编号:1208085MH157)
【分类号】：R687

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