D3-肌酸稀释法在小鼠骨骼肌质量检测中的应用

发布时间：2018-04-26 19:02

本文选题：D-肌酸 + 骨骼肌　；参考：《解放军医学杂志》2017年07期

【摘要】：目的探讨能否利用D3-肌酸稀释法检测小鼠骨骼肌质量,为小鼠骨骼肌质量检测提供新的方法。方法选择4周龄、10周龄和13月龄的清洁级雄性C57BL/6小鼠各10只,经Echo MRITM小动物体成分分析仪检测体成分后单次给予D3-肌酸(2mg/kg)灌胃,于24、48h和72h收集小鼠尿液,采用液相色谱串联质谱法(LC-MS/MS)检测尿液中D3-肌酸和D3-肌酐的含量,计算肌酸池大小,并对其与Echo MRITM检测的瘦体重进行相关性分析。结果 Echo MRITM测量的瘦体重及D3-肌酸稀释法得出的肌酸池均为老年鼠成年鼠幼年鼠。相关性分析显示,D3-肌酸稀释法计算得出的总体肌酸池与Echo MRITM测量的瘦体重呈显著正相关(r=0.687,P=0.000)。结论 D3-肌酐稀释法可用于检测小鼠骨骼肌质量。
[Abstract]:Objective to explore whether D 3 creatine dilution method can be used to detect the skeletal muscle mass of mice and to provide a new method for the detection of skeletal muscle quality in mice. Methods 10 clean male C57BL/6 mice of 4 weeks old and 10 weeks old and 13 months old male C57BL/6 mice were selected. The body composition was detected by Echo MRITM small animal composition analyzer, and then D3-creatine 2 mg / kg was given intragastrically. Urine samples were collected at 24 hours for 48 hours and 72 hours. Liquid chromatography-tandem mass spectrometry (LC-MS / MS) was used to detect the contents of D3-creatine and D3-creatinine in urine, and the size of creatine pool was calculated, and the correlation analysis was carried out between the contents of creatine pool and the lean body weight measured by Echo MRITM. Results the lean body weight measured by Echo MRITM and the creatine pool obtained by D 3 creatine dilution were all adult and juvenile rats. The correlation analysis showed that the total creatine pool calculated by D3-creatine dilution method was positively correlated with the lean body weight measured by Echo MRITM. Conclusion D 3 creatinine dilution method can be used to detect skeletal muscle mass in mice.
【作者单位】：锦州医科大学武警总医院研究生培养基地;汕头大学医学院药理学教研室;北京大学实验动物中心;
【基金】：科技部重大仪器专项(2011YQ030114) 武警总医院院级课题(WZ2016019)~~
【分类号】：R68

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