保留残端与不保留残端重建前交叉韧带腱骨愈合情况比较

发布时间：2018-04-29 01:11

本文选题：腱 + 前交叉韧带　；参考：《中国组织工程研究》2016年51期

【摘要】：背景:保留残端重建能够促进腱骨愈合,但目前的研究仅停留组织学水平。从分子生物层面报道保残重建对促进腱骨愈合作用机制的研究国内外尚未见报道。目的:探讨保留残端重建前交叉韧带对腱骨愈合的机制。方法:72只新西兰兔随机分为不保残组、牵张保残组和残端鞘内重建组,各24只,分别采取不保残、牵张保残和残端鞘内重建方法进行急性期双膝前交叉韧带重建。结果与结论:1苏木精-伊红染色结果显示,牵张保残组和残端鞘内重建组兔腱骨愈合优于不保残组,残端鞘内重建组优于牵张保残组;2实时荧光定量PCR结果表明,牵张保残组和残端鞘内重建组兔移植腱周边骨组织中骨保护素m RNA表达水平及骨保护素/RANKL比值大于不保残组,且残端鞘内重建组兔移植腱周边骨组织中上述指标大于牵张保残组,而RANKL m RNA表达水平小于不保残组;3结果提示2种保留残端重建前交叉韧带方法都能够促进腱骨愈合,其中以残端鞘内重建最明显,作用机制可能与早期上调骨道中骨保护素m RNA,下调RANKL m RNA有关。
[Abstract]:Background: preservation of stump reconstruction can promote tendon healing, but the present study only focuses on histology. The mechanism of residual preservation and reconstruction in promoting tendon healing has not been reported at home and abroad. Objective: to investigate the mechanism of reconstructing anterior cruciate ligament (ACL) with preservation of stump for tendon bone healing. Methods Twenty two New Zealand rabbits were randomly divided into three groups: the control group, the distraction group and the residual intrathecal reconstruction group, each with 24 rabbits. The anterior cruciate ligament (ACL) was reconstructed with the methods of non-preservation, distraction and intrathecal reconstruction, respectively. Results and conclusion the results of hematoxylin-eosin staining showed that the healing of tendon bone in the distraction preserving group and in the residual intrathecal reconstruction group was superior to that in the unpreserved group, and that in the stump intrathecal reconstruction group was superior to that in the distraction preserved remnant group by real-time fluorescence quantitative PCR. The expression level of osteoprotegerin RNA and the ratio of osteoprotegerin / RANKL in the bone tissue around the allograft tendon in the distraction preservation group and the residual intrathecal reconstruction group were higher than those in the unpreserved group. The results showed that the above indexes were higher in the grafted tendon peripheral bone tissue than those in the distraction group, and the RANKL m RNA expression level was lower than that in the unpreserved group. The results indicated that the two methods of preserving the stump and reconstructing the anterior cruciate ligament could promote the tendon bone healing. Among them, the stump intrathecal reconstruction is the most obvious, and the mechanism may be related to the up-regulation of osteoprotegerin RNAs and down-regulation of RANKL m RNA in the early stage.
【作者单位】：新疆医科大学附属肿瘤医院骨与软组织科;新疆武警兵团指挥部医院骨科;新疆医科大学附属肿瘤医院病理科;新疆医科大学第一附属医院骨科中心;
【基金】：新疆生产建设兵团自然科学基金资助项目(2015AD004)~~
【分类号】：R687.4

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