CTRP12血浆水平在冠心病中的变化及与七氟醚关系研究

发布时间：2018-04-30 10:43

  本文选题：CTRP12 + 冠心病　； 参考：《山西医科大学》2016年硕士论文

【摘要】：目的:通过本研究揭示CTRP12在CAD中的变化及与七氟醚的关系筛查CTRP12作为预示冠心病临床检测指标的可能性,并明确CTRP12的心脏保护效应。方法:随机选取健康体检志愿者62例为对照组(N组),选取心内科经冠脉造影证实CAD患者67例为冠心病组(C组),选取心外科预备行非体外循环的冠脉旁路移植术(OPCABG)患者52例分为丙泊酚组(P组)和丙泊酚+七氟醚组(S组),所有受试者均需禁食8小时以上(手术组还需同时禁饮6至少小时)并于次日清晨采集静脉血3~5ml,P组和S组须另外采集术后4h静脉血各5ml,设定在1000转/分的转速离心15分钟,用移液枪吸取上层血浆,分装放入设定温度在-70℃的冰箱冻存。记录N组、C组的年龄、性别、吸烟率、饮酒率、糖尿病患病率、高血压患病率、BMI、TC、TG、HDL-C、LDL-C、FBG以及CRP,并采用酶联免疫吸附法检测C组和N组的CTRP12、IL-1β;采用酶联免疫吸附法检测P组和S组术前P0、S0及术后P1、S1四个时间点的CTRP12浓度,并计算P0和P1,S0和S1的差值dP和dS。结果:所有数据用SPSS软件分析。结果得出冠心病组CTRP12与降低的IL-1β、HDL-C和增高的BMI呈正相关。在N、C、P、S组受试对象中CTRP12血浆水平与高血压患病率和BMI呈正相关,与HDL-C和IL-1β呈负相关。logistic回归分析CTRP12是冠心病的独立危险因素。与N组相比C组CTRP12浓度明显升高,且两者的差异有统计学意义。S组和P组术前CTRP12浓度无差异,P组CTRP12浓度在诱导前与术后4小时的差值dP低于S组诱导前与术后4小时的差值dS,且具有统计学差异。结论:目前的研究显示,血清CTRP12浓度可以为预测冠心病患病率提供参考。七氟醚的心肌保护作用可能与CTRP12有关。
[Abstract]:Objective: to investigate the changes of CTRP12 in CAD and the relationship between CTRP12 and sevoflurane (sevoflurane) as a predictor of coronary heart disease (CHD), and to determine the cardioprotective effect of CTRP12. Methods: 62 healthy volunteers were randomly selected as control group, 67 patients with CAD confirmed by coronary angiography in cardiology were selected as group C, and cardiac surgery was performed with off-pump coronary artery bypass grafting (OPCABG). 52 patients were divided into propofol group (group P) and propofol sevoflurane group (group S). All subjects had to fast for more than 8 hours (the operation group had to stop drinking for at least 6 hours at the same time) and collected venous blood in the next morning. Group A should collect 5 ml venous blood 4 hours after operation and centrifuge for 15 minutes at a rotational speed of 1000 rpm / min. Drain the upper plasma with a liquid-shifting gun and store in a refrigerator with a set temperature of-70 鈩，

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