血浆miR-92a与骨肉瘤预后相关性研究

发布时间：2018-05-03 16:09

本文选题：骨肉瘤 + mi　；参考：《中国癌症杂志》2016年08期

【摘要】：背景与目的:骨肉瘤是儿童和年轻成人最常见的原发性恶性骨肿瘤,目前尚无高特异度、高灵敏度的预后预测指标。该研究旨在探讨血浆mi R-92a表达水平与骨肉瘤临床病理及预后的关系。方法:收集30例健康体检者和45例骨肉瘤患者术前及术后血浆,通过实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)检测血浆mi R-92a水平。以术前mi R-92a≥5为界限,将骨肉瘤患者分为高表达组和低表达组。结果:骨肉瘤患者血浆mi R-92a水平显著高于正常体检人群,患者术前mi R-92a水平远高于术后。患者术前mi R-92a表达水平与患者的性别、年龄、肿瘤部位、肿瘤大小和组织学类型等无关,而与Enneking分期、肿瘤坏死率和肺转移相关。Kaplan-Meier分析高表达mi R-92a的骨肉瘤预后差(P=0.035)。结论:骨肉瘤患者血浆mi R-92a表达水平与生存率相关,可能是骨肉瘤一项有价值的预后指标。
[Abstract]:Background & objective: osteosarcoma is the most common primary malignant bone tumor in children and young adults. The aim of this study was to investigate the relationship between the expression of plasma mi-92a and clinicopathology and prognosis of osteosarcoma. Methods: plasma samples were collected from 30 healthy controls and 45 patients with osteosarcoma before and after operation. The plasma miR-92a levels were measured by real-time fluorescent quantitative polymerase chain reaction- RTFQ-PCRs. The patients with osteosarcoma were divided into high expression group and low expression group. Results: the plasma miR-92a level in patients with osteosarcoma was significantly higher than that in normal controls. The expression of miR-92a was not correlated with sex, age, tumor location, tumor size and histological type, but with Enneking stage, tumor necrosis rate and lung metastasis. Kaplan-Meier analysis showed that the prognosis of osteosarcoma with high expression of mi R-92a was 0.035%. Conclusion: the expression of miR-92a in patients with osteosarcoma is correlated with the survival rate, which may be a valuable prognostic index for osteosarcoma.
【作者单位】：南方医科大学基因工程研究所;解放军535医院介入科;南方医科大学南方医院创伤骨科;深圳市南山区妇幼保健院;
【分类号】：R738.1

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