脂肪乳剂逆转布比卡因心肌毒性中线粒体凋亡途径相关蛋白的变化

发布时间：2018-05-06 22:13

本文选题：线粒体 + 细胞凋亡　；参考：《临床麻醉学杂志》2017年06期

【摘要】：目的检测脂肪乳剂逆转布比卡因心肌毒性作用中线粒体凋亡通路相关蛋白的变化,探讨脂肪乳剂逆转布比卡因心脏毒性的可能机制。方法日龄1~3d的健康SD大鼠乳鼠,取其心室肌进行体外原代培养,用含有布比卡因的培养基培养24h来建立心肌细胞布比卡因中毒模型。实验分组:空白对照组(C组)、布比卡因组(B组)、布比卡因+脂肪乳剂组(BL组)。采用流式细胞仪检测细胞凋亡情况,采用Western blot法检测细胞内细胞色素C(cytochrome C,Cyto-C)和活化的casepase-3蛋白含量。结果与C组比较,B组和BL组细胞凋亡率明显升高,且B组明显高于BL组(P0.05)。与C组比较,B组和BL组Cyto-C和活化的casepase-3含量均明显升高(P0.05),且B组明显高于BL组(P0.05)。结论脂肪乳剂可能通过抑制线粒体Cyto-C释放,降低casepase-3激活来调节细胞凋亡,从而发挥保护心肌细胞的作用。
[Abstract]:Objective to detect the changes of mitochondrial apoptotic pathway related proteins in lipid emulsion reversing myocardial toxicity of bupivacaine and to explore the possible mechanism of lipid emulsion reversing cardiac toxicity of bupivacaine. Methods the ventricular muscles of healthy SD rats were cultured in vitro and cultured in vitro for 24 hours in order to establish the model of bupivacaine poisoning in cardiomyocytes. The experiment was divided into three groups: control group (C), bupivacaine group (B), bupivacaine fat emulsion group (BL). Apoptosis was detected by flow cytometry, cytochrome C(cytochrome (Cyto-C) and activated casepase-3 protein were detected by Western blot assay. Results compared with group C, the apoptosis rate of B group and BL group was significantly higher than that of BL group (P 0.05). Compared with group C, the contents of Cyto-C and activated casepase-3 in group B and group BL were significantly higher than those in group B and group B (P 0.05) and higher than that in group B (P 0.05). Conclusion Fat emulsion may regulate cell apoptosis by inhibiting mitochondrial Cyto-C release and casepase-3 activation, thus protecting cardiomyocytes.
【作者单位】：潍坊医学院麻醉学系;潍坊医学院临床医学系;
【基金】：山东省自然科学基金(ZR2012HM080) 潍坊医学院科技创新基金(K1302009)
【分类号】：R614

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