大蒜素对兔膝骨关节炎作用的实验研究

发布时间：2018-05-07 18:10

本文选题：大蒜素 + 骨关节炎　；参考：《中国矫形外科杂志》2017年15期

【摘要】：[目的]探讨大蒜素对兔膝骨关节炎的作用。[方法]将30只家兔随机分为空白对照组、模型对照组和大蒜素组,每组10只;除空白对照组外其余2组按改良Hulth法建立模型,术后开始灌服药物,大蒜素组(大蒜素,128 mg/kg/d)、空白对照组和模型对照组(等量生理盐水20 ml),连用8周。术后8周处死所有家兔取右膝股骨髁大体观察;苏木精-伊红(HE)和番红O(SA-O)染色对股骨髁关节软骨行Mankin评分;采用q RTPCR法检测股骨内侧髁负重区软骨组织interleukin-1β(白细胞介素-1β,IL-1β)、matrix metalloproteinase-3(基质金属蛋白酶-3,MMP-3)和tissue inhibitor of metalloproteases-1(基质金属蛋白酶组织抑制因子-1,TIMP-1)的m RNA相对表达量。[结果]三组之间比较,空白对照组的Mankin评分最低,IL-1β、MMP-3和TIMP-1 m RNA表达量最低,模型对照组和大蒜素组,分别与空白对照组比较,上述指标差异均有统计学意义(P0.05);与模型对照组比较,大蒜素组Mankin评分降低,IL-1β、MMP-3、TIMP-1 m RNA表达量降低,差异有统计学意义(P0.05)。[结论]大蒜素能够改善兔膝骨关节炎Mankin评分并能够下调股骨髁关节软骨中IL-1βm RNA、MMP-3m RNA表达,上调TIMP-1 m RNA表达量。
[Abstract]:[objective] to investigate the effect of allicin on knee osteoarthritis in rabbits. [methods] Thirty rabbits were randomly divided into blank control group, model control group and allicin group with 10 in each group. Allicin group (allicin 128mg / kg / d), blank control group and model control group (20 ml saline, 8 weeks). Eight weeks after operation, all the rabbits were sacrificed for gross observation of the femoral condyle of the right knee, and the Mankin scores of the femoral condylar cartilage were evaluated by hematoxylin eosin (HEH) and guanghong (OSA-O) staining. The expression of interleukin-1 尾 (IL-1 尾) matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteases-1 (TIMP-1) in cartilage of the medial condylar of femur were detected by Q RTPCR method. [results] the expression of IL-1 尾 -MMP-3 and TIMP-1 m RNA in the blank control group was the lowest, and that in the model control group and allicin group was the lowest, respectively, compared with the blank control group. Compared with the model control group, the Mankin score of allicin group was lower than that of the model control group, and the expression of IL-1 尾, MMP-3, TIMP-1 m RNA was significantly lower in allicin group than that in the model control group (P 0.05). [conclusion] allicin can improve the Mankin score of rabbit knee osteoarthritis, down-regulate the expression of IL-1 尾 -RNA-3m RNA and up-regulate the expression of TIMP-1 m RNA in femoral condylar articular cartilage.
【作者单位】：遵义医学院附属医院关节外科;
【基金】：贵州省科技厅社发攻关基金资助项目(编号:黔科合SY[2010]3091号) 硕士启动基金项目(编号:院字[2013]20号)
【分类号】：R684.3

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