白芦藜醇对骨肉瘤细胞U-2OS增殖和凋亡的影响及其分子机制

发布时间：2018-05-07 23:11

本文选题：白芦藜醇 + 骨肉瘤细胞U-OS　；参考：《中国生物制品学杂志》2016年01期

【摘要】：目的观察白芦藜醇(resveratrol,Res)对骨肉瘤细胞U-2OS增殖和凋亡的影响,并探讨其可能的分子机制。方法体外培养人骨肉瘤细胞U-2OS,用不同浓度的Res(5、10、20、40、80μmol/L)作用不同时间(24、48、72 h)后,MTT法检测Res对U-2OS细胞增殖活力的影响;流式细胞术检测Res对细胞周期和凋亡的影响;应用Caspase-3活性检测试剂盒检测Caspase-3酶活性;Western blot检测细胞内Bax、Bcl-2和Caspase 3蛋白的表达水平。结果 Res作用24 h时,与空白对照组及DMSO组比较,20和40μmol/L Res组的细胞增殖抑制率、G0/G1和G2/M期的细胞比例、细胞凋亡率、Caspase-3酶活性显著升高(P0.05),S期细胞比例显著降低(P0.05);20μmol/L Res组U-2OS细胞中Bax和Caspase-3蛋白表达水平显著升高(P0.05),而Bcl-2蛋白表达水平明显降低(P0.05)。结论 Res通过诱导提高Caspase-3酶活性,上调促凋亡基因Bax、Caspase-3及下调抗凋亡基因Bcl-2蛋白的表达水平,从而抑制U-2OS细胞的增殖,促进其凋亡,为Res治疗骨肉瘤提供了实验依据。
[Abstract]:Objective to observe the effect of Chenopodium alopecuroides on the proliferation and apoptosis of osteosarcoma cell line U-2OS, and to explore its possible molecular mechanism. Methods Human osteosarcoma cells U-2OS were cultured in vitro and treated with different concentrations of Resch51010 ~ (20) ~ 4080 渭 mol / L for different time, respectively. The effects of Res on the proliferation and apoptosis of U-2OS cells were detected by flow cytometry (FCM), and the effects of Res on cell cycle and apoptosis were detected by flow cytometry (FCM), and the proliferation of human osteosarcoma cells was determined by flow cytometry (FCM). Caspase-3 activity assay kit was used to detect the activity of Caspase-3. Western blot was used to detect the expression of Bcl-2 and Caspase 3 in cells. Results after treatment with Res for 24 h, compared with control group and DMSO group, the inhibition rate of cell proliferation in 20 渭 mol/L Res group and 40 渭 mol/L Res group was compared with that in G 0 / G 1 and G 2 / M phase. Apoptosis rate and activity of Caspase-3 increased significantly. The proportion of P0.05An S phase cells decreased significantly. The expression of Bax and Caspase-3 protein increased significantly in the U-2OS cells of 20 渭 mol/L Res group, while the expression level of Bcl-2 protein decreased significantly. Conclusion Res can increase the activity of Caspase-3, up-regulate the expression of apoptosis-promoting gene Bax-Caspase-3 and down-regulate the expression of anti-apoptotic gene Bcl-2, so as to inhibit the proliferation of U-2OS cells and promote its apoptosis, which provides an experimental basis for the treatment of osteosarcoma by Res.
【作者单位】：达州市中心医院骨科;重庆医科大学神经科学研究中心;
【分类号】：R738.1

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