西黄丸对兔膝假体置入后感染表皮葡萄球菌细菌生物膜的影响

发布时间：2018-05-10 14:27

本文选题：西黄丸 + 细菌生物膜　；参考：《南华大学》2015年硕士论文

【摘要】：目的1、研究西黄丸离体环境中对表皮葡萄球菌BF的影响作用;2、将西黄丸应用于兔建立的人工膝关节假体表皮葡萄球菌感染模型,研究西黄丸在体内环境中对表皮葡萄球菌BF的影响作用。方法1、西黄丸在离体环境中对表皮葡萄球菌BF影响的实验研究通过检测表皮葡萄球菌株ATCC35984 BF形成的能力,观察西黄丸对表皮葡萄球菌胞外粘质物(ESS)产生的影响作用;采用荧光显微镜、激光共聚焦显微镜观察BF形态的方法,研究西黄丸在离体环境中对表皮葡萄球菌BF的影响作用。2、西黄丸体内环境中表皮葡萄球菌BF影响的实验研究采用新西兰大白兔来建立膝关节人工假体周围感染表皮葡萄球菌BF模型:术后1周注射表皮葡萄球菌株ATCC35984于大白兔膝关节腔内,建立人工关节假体感染模型。接种后1周并确认感染造模成功后,随机将实验动物分成4组,分别为西黄丸组(Xi Huang Wan group,XG)、左氧氟沙星组(Levofloxacin group,LG)、联合组(Joint group,JG)及空白组(Control group,CG),通过喂饲给药,观察动物全身情况,检测WBC、ESR、CRP含量,对假体周围表面BF细菌计数、采用激光共聚焦显微镜观察BF形态,并进行关节内细菌培养、病理切片等,观察西黄丸对人工关节假体周围表皮葡萄球菌感染BF的影响作用。结果1、体外实验显示表皮葡萄球菌株ATCC35984有较强能力形成BF;西黄丸可减少表皮葡萄球菌株ATCC35984BF分泌ESS,西黄丸能协同增强左氧氟沙星对表皮葡萄球菌BF内细菌的杀灭作用。2、行干预治疗后,XG、LG及JG的动物BF均有减少,而以JG最明显。体内试验显示西黄丸联合左氧氟沙星可有效预防和治疗关节假体表皮葡萄球菌感染,西黄丸能协同增强左氧氟沙星杀菌作用。结论1、离体环境试验显示西黄丸能抑制表皮葡萄球菌BF生成。2、体内环境试验显示西黄丸能抑制表皮葡萄球菌BF的形成,与左氧氟沙星合用能增强抗感染的效果。
[Abstract]:Objective 1 to study the effect of Xihuang Pill on Staphylococcus epidermidis BF in vitro and to apply Xihuang Pill to the rabbit model of staphylococcus epidermidis infection with artificial knee prosthesis. To study the effect of Xihuang pill on the BF of Staphylococcus epidermidis in vivo. Methods 1. The effect of Xihuang Pill on ATCC35984 BF of Staphylococcus epidermidis in vitro was studied. The effect of Xihuang Pill on the production of extracellular mucus of Staphylococcus epidermidis was observed by detecting the ability of forming Staphylococcus epidermidis strain ATCC35984 BF; The morphology of BF was observed by fluorescence microscope and confocal laser microscope. To study the effect of Xihuang Pill on the BF of Staphylococcus epidermidis in Vitro and the effect of Staphylococcus epidermidis BF in Vitro the New Zealand White Rabbit was used to establish the infection table around artificial prosthesis of knee joint. Staphylococcus epidermidis BF model: staphylococcus epidermidis strain ATCC35984 was injected into the knee joint cavity of rabbits 1 week after operation. To establish artificial joint prosthesis infection model. After 1 week of inoculation, the animals were randomly divided into 4 groups: Xi Huang Wan group XGG group, levofloxacin group (Levofloxacin group), joint group (JGG) and control group (CGG), respectively. The content of ESR-CRP was measured, the BF bacteria around the prosthesis were counted, the morphology of BF was observed by confocal laser microscope, and the bacteria culture and pathological section were performed. To observe the effect of Xihuang Pill on BF infection of Staphylococcus epidermidis around artificial joint prosthesis. Results 1. In vitro experiments showed that Staphylococcus epidermidis strain ATCC35984 had a strong ability to form BF.The Xihuang pills could reduce the secretion of ESSs from Staphylococcus epidermidis strain ATCC35984BF, and Xihuang pills could enhance the killing effect of levofloxacin on bacteria in Staphylococcus epidermidis BF. The BF of both XGG LG and JG decreased after intervention. JG is the most obvious. In vivo tests showed that Xihuang Pill combined with levofloxacin could effectively prevent and treat staphylococcus epidermidis infection in prosthetic joint, and Xihuang pills could enhance the bactericidal effect of levofloxacin. Conclusion 1. In vitro environmental tests showed that Xihuang pills could inhibit the BF production of Staphylococcus epidermidis. In vivo environmental tests showed that Xihuang pills could inhibit the formation of Staphylococcus epidermidis BF and the combination with levofloxacin could enhance the effect of anti-infection.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.4

【相似文献】