肝线粒体相关内质网膜在袖状胃切除术改善糖尿病大鼠胰岛素敏感性中的作用

发布时间：2018-05-10 15:42

本文选题：袖状胃切除术 + 线粒体相关内质网膜　；参考：《山东大学学报(医学版)》2017年10期

【摘要】：目的探讨袖状胃切除术(SG)对糖尿病大鼠肝脏线粒体相关内质网膜(MAMs)的影响及其机制。方法建立Wistar大鼠糖尿病模型,选取20只随机分为SG手术组(n=10)和SHAM假手术组(n=10),测量两组大鼠术前术后的体质量、进食量以及胰岛素敏感性变化,并在术后12周取大鼠肝脏检测MAMs中1,4,5-三磷酸肌醇受体(IP3R)、线粒体融合蛋白2(Mfn2)、葡萄糖调节蛋白75(Grp75)、线粒体钙单向转运体(MCU)等蛋白分子的表达量变化。结果与SHAM组比较,SG组术后4周起进食量减少,6周起体质量降低;术后第2、12周,口服葡萄糖耐量试验曲线下面积、胰岛素耐量试验曲线下面积、稳态模型评估胰岛素抵抗明显减少;SG组术后MAMs中与钙离子平衡相关的IP3R、MCU及与内质网线粒体连接相关的Mfn2等蛋白表达量均低于SHAM组(P0.05)。结论糖尿病大鼠SG术后胰岛素的敏感性改善可能与术后MAMs钙离子通道相关蛋白的表达下降有关。
[Abstract]:Objective to investigate the effect and mechanism of sleeve gastrectomy (SGG) on hepatic mitochondria associated endoplasmic omentum (MAMs) in diabetic rats. Methods the diabetic model of Wistar rats was established. Twenty rats were randomly divided into SG operation group (n = 10) and SHAM sham operation group (n = 10). The body weight, food intake and insulin sensitivity were measured before and after operation in two groups. At 12 weeks after operation, the expression levels of 1T 4N 5 inositol receptor (IP3RN), mitochondrial fusion protein 2mfn2, glucose regulated protein 75 GRP 75, mitochondrial calcium one-way transporter (MCU) and so on were detected in the liver of rats. Results compared with SHAM group, the body mass of SG group decreased from 4 weeks to 6 weeks, the area under oral glucose tolerance test curve and the area under insulin tolerance test curve were decreased at 2 ~ 12 weeks after operation. The steady-state model showed that insulin resistance significantly reduced the expression of Ca ~ (3 +) -related IP3RN MCU and Mfn2 associated with endoplasmic reticulum mitochondria in SG group compared with SHAM group (P 0.05). Conclusion the improvement of insulin sensitivity after SG in diabetic rats may be related to the decrease of MAMs calcium channel associated protein expression.
【作者单位】：山东大学齐鲁医院普通外科;
【基金】：国家自然科学基金(81370496) 山东大学基本科研业务费专项基金(2014QLKY22)
【分类号】：R587.1;R656.61

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