异泽兰黄素抑制瘢痕组织成纤维细胞增殖的机制

发布时间：2018-05-10 17:53

本文选题：异泽兰黄素 + 瘢痕成纤维细胞　；参考：《中国老年学杂志》2017年22期

【摘要】：目的探讨异泽兰黄素对瘢痕组织成纤维细胞增殖的影响及机制。方法应用MTT法检测异泽兰黄素对瘢痕组织成纤维细胞活力的影响,流式细胞仪检测异泽兰黄素对瘢痕组织成纤维细胞中活性氧(ROS)的生成及细胞凋亡的影响,Western印迹法检测异泽兰黄素对细胞内JNK和Bcl2磷酸化的影响。结果 2.5、5.0、10.0、20.0、40.0μg/ml异泽兰黄素作用细胞24 h后,细胞活力呈浓度依赖性降低。ROS抑制剂(NAC)预处理细胞1 h能显著抑制异泽兰黄素对细胞活力的抑制作用。10.0μg/ml异泽兰黄素作用细胞24 h能促进细胞ROS生成和细胞凋亡,细胞凋亡率为(47.20±1.60)%,与正常对照组相比具有显著统计学差异;促进JNK磷酸化水平和Bcl2表达水平,JNK磷酸化水平为(3.12±0.25)倍,Bcl2磷酸化水平为(2.84±0.22)倍,与正常对照组相比具有显著性差异。JNK磷酸化抑制剂和NAC均能显著抑制异泽兰黄素诱导的细胞凋亡和JNK的磷酸化。结论异泽兰黄素可诱导瘢痕组织成纤维细胞ROS的产生,通过激活JNK/Bcl2通路诱导细胞凋亡,从而抑制成千维细胞的增殖活性。
[Abstract]:Objective to investigate the effect and mechanism of isoflavin on the proliferation of scar tissue fibroblasts. Methods MTT was used to detect the effect of isoflavin on the activity of fibroblasts in scar tissue. Flow cytometry was used to detect the effect of isoflavin on the formation of reactive oxygen species (ROS) and apoptosis in scar tissue fibroblasts, Western blot The effect of isoflavin on the phosphorylation of JNK and Bcl2 in cells. Results the activity of 2.5,5.0,10.0,20.0,40.0 mu g/ml isoremaflavin was 24 h, and the cell viability was reduced to a concentration dependent.ROS inhibitor (NAC) pretreated cell 1 h can significantly inhibit the inhibitory effect of isislavin on cell viability,.10.0 micron g/ml isoeugenin Cell 24 h can promote cell ROS production and cell apoptosis, the rate of cell apoptosis is (47.20 + 1.60)%, compared with normal control group, there is significant statistical difference. The level of phosphorylation of JNK and Bcl2 expression level, JNK phosphorylation level is (3.12 + 0.25) times, Bcl2 phosphorylation level is (2.84 + 0.22) times, compared with the normal control group has significant difference. .JNK phosphorylation inhibitors and NAC can significantly inhibit the apoptosis induced by isoflavin and the phosphorylation of JNK. Conclusion ISO zisan yellow can induce the production of ROS in scar tissue fibroblasts, and induce apoptosis by activating JNK/Bcl2 pathway, thus inhibiting the proliferation activity of thousands of cells.

【作者单位】：广东医科大学附属医院整形外科;暨南大学附属第一医院整形科;
【基金】：国家自然科学基金资助项目(81372065)
【分类号】：R622

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