二甲氨基乙醇和复方氨基酸溶液对衰老大鼠皮肤的Periostin和Dermatopontin表达的影响

发布时间：2018-05-12 12:03

本文选题：二甲氨基乙醇 + 复方氨基酸　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:根据中胚层疗法(Mesotherapy)理念,观察皮肤真皮内注射二甲氨基乙醇(DMAE)和复方氨基酸溶液(AA)对D-半乳糖(D-gal)诱导的化学性衰老大鼠皮肤的成骨细胞特异性因子(Periostin,POSTN)和皮肤桥蛋白(Dermatopontin,DPT)表达的影响。方法:使用80只Wistar大鼠,入组随机,按照正常对照组、衰老对照组、衰老治疗组。注射D-半乳糖后第18天开始,衰老治疗组在大鼠两侧臀部皮肤分别注射0.1%DMAE+AA、0.2%DMAE+AA、0.1%DMAE、0.2%DMAE、AA、NS,每周1次,连续4周。观察各组皮肤组织学变化,真皮厚度、羟脯氨酸(Hydroxyproline,Hyp)、I型及III型胶原蛋白m RNA表达、成骨细胞特异性因子(Periostin,POSTN)和皮肤桥蛋白(Dermatopontin,DPT)表达水平的变化。结果:在衰老大鼠真皮内注射0.1%DMAE+AA、0.2%DMAE+AA,可促进老化大鼠真皮厚度、Hyp含量和I和III型胶原蛋白m RNA及POSTN表达增加,而对DPT表达没有影响。结论:在衰老大鼠中注射DMAE和AA复合溶液,能有效改善衰老的皮肤组织结构与已老化且变性的胶原蛋白。同时能是促进衰老大鼠皮肤进行胶原蛋白合成,并明显增加皮肤真皮厚度及胶原蛋白含量,对抗衰老具有一定延缓老化皮肤的作用。
[Abstract]:Objective: according to the idea of mesodermic therapy, To observe the effects of intradermal injection of dimethylaminoethanol (DMAE) and compound amino acid solution (AAA) on the expression of D-galactose-induced osteoblast specific factor Periostinpost (POSTN) and dermatopontinine (DPTT) in the skin of aged rats. Methods: 80 Wistar rats were randomly divided into two groups: normal control group and aging treatment group. From the 18th day after the injection of D-galactose, the aging treatment group received 0.2DMAE 0.2DMAE 0.2DMAE 0.2DMAEA NSS once a week for 4 weeks in the aging treatment group. The changes of skin histological changes, dermal thickness, the expression of collagen type I and type III collagen m RNA, the osteoblast specific factor Periostin post and the expression level of Dermatopontintinin were observed. Results: injection of 0.1%DMAE AA0.2DMAE into dermis of aging rats increased the content of Hyp and the expression of type I and III collagen m RNA and POSTN in the dermis of aging rats, but had no effect on the expression of DPT. Conclusion: the compound solution of DMAE and AA can effectively improve the aging skin tissue structure and the aging and denatured collagen in aging rats. At the same time, it can promote collagen synthesis in the skin of aging rats, and increase the dermal thickness and collagen content of the skin, which has the effect of delaying aging skin.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R62

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