高频正弦波振动抑制废用性肌萎缩的凋亡进程

发布时间：2018-05-12 23:06

本文选题：高频正弦波振动 + 肌梭　；参考：《成都体育学院学报》2017年02期

【摘要】：目的:探讨高频正弦波振动(HFV)对骨骼肌在废用状态时细胞凋亡的对抗作用。方法:建立废用动物模型,改进振动仪的关键技术,采用短时间歇式振动的方法,结合电镜观察、免疫组化染色法和TUNEL法检测,观察HFV对制动大鼠比目鱼肌(SOL)湿重体重比、肌纤维横截面积、肌纤维超微结构的变化、Bcl-2和Bax的表达情况。结果:(1)制动后大鼠SOL湿重体重比为30.22±2.68,下降了36.43%。肌纤维的横截面积为3789.56±95.14μm2,下降了37.77%。HFV组相对制动组的SOL湿重体重比及横截面积均有所增加(P0.05);(2)电镜可见制动后梭外肌病变明显,核内染色质团块状凝集。梭内肌部分肌膜溶解消失,细胞核变形。振动组的梭外肌Z线结构比较清晰,梭内肌纤维结构未见明显改变;(3)制动后大鼠SOL中Bcl-2阳性表达率为20%,明显低于对照组的表达情况(P0.05)。制动后Bax阳性表达率为80%,明显高于对照组的表达情况(P0.05)。制动后AI为20%,明显高于对照组(P0.05)。HFV组相对制动组的Bcl-2阳性表达率均有所增加,Bax阳性表达率以及AI有所降低(P0.05)。结论:改进后的抗肌萎缩振动仪操作性较强,对制动大鼠废用性肌萎缩的凋亡进程有抑制作用。
[Abstract]:Aim: to investigate the effect of high frequency sine wave vibration (HFV) on apoptosis of skeletal muscle. Methods: to establish a waste animal model, improve the key technology of vibrator, observe the wet weight / body weight ratio of HFV to soleus muscle of brake rats by using the method of short-term intermittent vibration, electron microscope observation, immunohistochemical staining and TUNEL method. The cross sectional area of muscle fiber and the changes of ultrastructure of muscle fiber were detected by the expression of Bcl-2 and Bax. Results the wet weight / body weight ratio of SOL was 30.22 卤2.68, which decreased by 36.43%. The cross sectional area of muscle fiber was 3789.56 卤95.14 渭 m ~ (2), which decreased the SOL wet weight / body weight ratio and cross-sectional area of relative immobilization group in 37.77%.HFV group. Part of the intrafusal muscle membrane dissolves and the nucleus deforms. In vibration group, the Z line structure of extrafusal muscle was clear, and the fiber structure of intrafusal muscle did not change significantly. The positive expression rate of Bcl-2 in SOL of rats after immobilization was 20%, which was significantly lower than that of control group (P 0.05). The positive expression rate of Bax after immobilization was 80%, which was significantly higher than that of control group (P 0.05). After immobilization, AI was 20%, which was significantly higher than that in the control group (P 0.05). The positive expression rate of Bcl-2 in the control group was higher than that in the control group. The positive expression rate of Bax was increased and the AI was decreased (P 0.05). Conclusion: the improved anti-atrophy vibrator has strong operability and can inhibit the process of apoptosis of disused muscle atrophy in immobilization rats.
【作者单位】：西安交通大学体育中心;西安交通大学第一附属医院;西安交通大学医学院;
【基金】：国家自然科学基金项目“从肌梭的改变探讨正弦波振动对抗废用性肌肉萎缩的作用机制”(30901583) 中央高校基本科研业务费专项资金资助西安交通大学基本科研业务费国际合作项目(1191320036)
【分类号】：R685

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