无锡市第三人民医院烧伤科病房环境细菌与烧伤病员创面细菌的同源性分析

发布时间：2018-05-14 14:11

本文选题：烧伤 + 细菌　；参考：《现代预防医学》2017年04期

【摘要】：目的对烧伤科病房环境细菌和烧伤病员创面细菌进行同源性分析,以判断是否存在耐药菌株的克隆性播散,为制定积极有效的消毒灭菌措施和控制感染传播提供依据。方法医院烧伤科病房于2012年8月28日搬迁,对搬迁前后新病房水池、病床护栏、床柜台面、心电监护仪按键及输液泵按键进行自2012年8月28日-9月5日为期5个采集日的采样、菌落计数并做细菌鉴定,同时也对病员创面进行5个采集日的同步骤操作。利用ERIC-PCR对采集的主要类型细菌进行同源性分析。结果在5个采集日内,烧伤科病房环境物体表面检出细菌数呈递增趋势,分离所得的20株鲍曼不动杆菌中18株的同源性为99.5%~100.0%,13株金黄色葡萄球菌中11株的同源性为100.0%。结论病房环境和病员创面的检出细菌间同源性很高,存在一定的交叉感染途径,有可能会造成院内感染的流行。
[Abstract]:Objective to analyze the homology of environmental bacteria in burn ward and wound bacteria in burn patients in order to determine whether there are drug resistant strains and to provide basis for making effective disinfection and sterilization measures and controlling the transmission of infection. Methods the hospital burn ward was moved on August 28, 2012. The new ward pool, bed guardrail, bed counter surface, ECG monitor key and infusion pump button were sampled from 28 August to 5 September 2012. The bacteria were counted and identified. At the same time, the wound was collected for 5 days. ERIC-PCR was used to analyze the homology of the main types of bacteria collected. Results the number of bacteria detected on the surface of environmental objects in the burn ward showed an increasing trend in 5 days. The homology of 18 of the 20 strains of Acinetobacter baumannii was 99.5%. The homology of 11 of the 13 strains of Staphylococcus aureus was 100.0%. Conclusion there is a high homology between bacteria detected in ward environment and wound, and there is a certain cross infection pathway, which may lead to the prevalence of nosocomial infection.
【作者单位】：无锡市第三人民医院;
【基金】：江苏省自然科学基金BK20141101
【分类号】：R644

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