富白细胞和血小板纤维蛋白凝胶对BMSCs在兔股骨头缺血性坏死中成骨作用的影响

发布时间：2018-05-14 19:55

本文选题：股骨头缺血性坏死 + 髓芯减压　；参考：《安徽医科大学学报》2017年01期

【摘要】：目的观察富白细胞和血小板纤维蛋白凝胶(L-PRF)联合骨髓间充质干细胞(BMSCs)在兔股骨头缺血性坏死(ANFH)试验中对成骨作用的影响。方法48只新西兰白兔随机分为A、B、C、D 4组(各组12只),均造双侧ANFH模型。后给予ANFH修复方法:A组单纯行髓芯减压;B组行髓芯减压联合L-PRF凝胶移植;C组行髓芯减压联合BM-SCs移植;D组行髓芯减压联合BMSCs和L-PRF凝胶移植。A、B、C、D组于术后4、8、12周摄X片后,每组随机选取4只行相关组织学检查、新生骨体积分数、新生血管计数、X射线评分。结果各组股骨头坏死中呈现不同程度的新骨形成。C、D组新骨的体积分数,血管计数及X射线评分较A、B组差异显著,其中D组比其余各组均高,B组强于A组,差异有统计学意义(P0.05)。结论L-PRF凝胶在医治兔AN-FH模型中对BMSCs成骨有促进作用,并拟使用L-PRF凝胶和BMSCs兼并髓芯减压治疗ANFH提供理论指导。
[Abstract]:Objective to observe the effect of leukocyte and platelet fibrin gel (L-PRF) combined with bone marrow mesenchymal stem cells (BMSCs) on osteogenesis in rabbits with avascular necrosis of femoral head (ANFH). Methods 48 New Zealand white rabbits were randomly divided into 4 groups (12 rabbits in each group) and bilateral ANFH models were established. ANFH repair method was given to ANFH repair method in group B and group B were treated with core decompression combined with L-PRF gel transplantation. Group C was treated with core decompression combined with BM-SCs transplantation. Group D was treated with core decompression combined with BMSCs and L-PRF gel transplantation. Group A was radiographed 12 weeks after operation. Each group was randomly selected to perform histological examination, bone volume fraction, neovascularization count and X-ray score. Results the volume fraction, blood vessel count and X-ray score of new bone in group D were significantly higher than those in group A (P 0.05), and that in group D was higher than that in group A (P 0.05). Conclusion L-PRF gel can promote the osteogenesis of BMSCs in rabbit AN-FH model and provide theoretical guidance for the treatment of ANFH with L-PRF gel and BMSCs combined with core decompression.
【作者单位】：安徽医科大学第一附属医院骨科;安徽医科大学第二附属医院骨科;
【基金】：国家自然科学基金(编号:81171173)
【分类号】：R681.8

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