白细胞介素1β对破骨细胞空泡型质子泵活性与胞内酸化的影响

发布时间：2018-05-16 04:23

本文选题：白细胞介素β + 空泡型质子泵　；参考：《中国病理生理杂志》2017年04期

【摘要】：目的:通过体外实验研究白细胞介素1β(IL-1β)对破骨细胞空泡型质子泵与细胞内pH值的影响,以探讨IL-1β促进破骨细胞骨吸收的机制。方法:骨髓单核巨噬细胞经过诱导成为破骨细胞后,予以0.1、0.3和0.5μg/L的IL-1β干预48 h,检测破骨细胞空泡型质子泵mRNA和蛋白的表达以及IL-1β对破骨细胞胞内酸化的影响,观察空泡型质子泵活性的改变与破骨细胞骨吸收功能的变化。结果:研究结果提示破骨细胞经IL-1β干预48 h后,空泡型质子泵的表达与活性均显著增加,并且细胞内pH值下降。破骨细胞与含有IL-1β的细胞培养液共同孵育后,其骨吸收能力增强,培养液中IL-1β的浓度越高,该作用越趋明显。结论:IL-1β可能是通过上调空泡型质子泵的表达与酶的活性,促进氢离子的产生和(或)转运,引发破骨细胞骨吸收活动增加,最终导致骨质破坏,由此参与骨关节炎症性疾病的发生。
[Abstract]:Objective: To study the effect of interleukin 1 beta (IL-1 beta) on the vacuolar proton pump and intracellular pH in osteoclast in vitro, in order to explore the mechanism of IL-1 beta to promote bone resorption of osteoclasts. Method: bone marrow mononuclear macrophages were induced to become osteoclasts, and 0.1,0.3 and 0.5 g/L IL-1 beta intervention 48 h were used to detect osteoclast empty cells. The expression of mRNA and protein in the bubble proton pump and the effect of IL-1 beta on the acidification of osteoclasts, observe the changes in the activity of the vacuolar proton pump and the changes of bone resorption function of the osteoclast. Results: the results suggest that after the intervention of 48 h by IL-1 beta, the expression and activity of the vacuolar proton pump are significantly increased, and the pH value in the cell is also observed. When the osteoclast is incubated with the cell culture fluid containing IL-1 beta, the bone absorption capacity of the cell is enhanced, the higher the concentration of IL-1 beta in the culture medium, the more obvious the effect. Conclusion: IL-1 beta may promote the production and (or) transport of hydrogen ions by up regulation of the expression of the vacuolar proton pump and the activity of the enzyme, causing the bone resorption activity of osteoclasts. The increase will eventually lead to bone destruction, which is involved in the occurrence of bone and joint inflammatory diseases.

【作者单位】：中山大学附属第五医院肾内科;中山大学附属第五医院普外科;
【基金】：广东省自然科学基金博士启动项目(No.2016A030310193)
【分类号】：R68

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