HIF-1α、VEGF和PCNA在退变性腰椎间盘中的表达及意义

发布时间：2018-05-19 08:20

本文选题：腰椎间盘退变 + 血管内皮生长因子　；参考：《皖南医学院》2015年硕士论文

【摘要】：目的:研究缺氧诱导因子-1α(HIF-1α)、血管内皮生因子(VEGF)和增殖细胞核抗原(PCNA)分别在纤维环完整型和纤维环破裂型腰椎间盘髓核组织的表达,探讨上述三种细胞因子的相关性。方法:采用腰背部正中后路手术,摘除符合实验条件的退行性腰椎间盘髓核组织标本共42例,其中人类腰椎间盘髓核组织纤维环完整型21例,人类腰椎间盘髓核组织的纤维环破裂型21例。运用HE染色在光镜下观察纤维环破裂型和纤维环完整型腰椎间盘髓核组织退变情况。采用免疫组化SP法检测缺氧诱导因子-1α(HIF-1α)、血管内皮生因子(VEGF)、增殖细胞核抗原(PCNA)分别在纤维环完整型和纤维环破裂型髓核组织中的表达情况并进行相关分析。结果:HE染色显示纤维环完整型髓核组织软骨细胞密度明显高于纤维环破裂型(P0.01),缺氧诱导因子-1α(HIF-1α)、血管内皮生因子(VEGF)、增殖细胞核抗原(PCNA)在纤维环完整型髓核组织中与纤维环破裂型髓核组织中均有表达,并且三种细胞因子在纤维环破裂型腰椎间盘髓核组织中的表达率明显高于纤维环完整型(P0.01)。VEGF与HIF-1α和VEGF与PCNA在退变性腰椎间盘髓核组织中的表达均高度相关。结论:腰椎间盘退变伴随了细胞密度的下降,纤维环破裂型腰椎间盘组织较纤维环完整型退变程度严重。随着腰椎间盘退变程度加剧,缺氧诱导因子-1α(HIF-1α)、血管内皮生因子(VEGF)、增殖细胞核抗原(PCNA)表达率增加,在退行性腰椎间盘髓核组织中HIF-1α与VEGF、VEGF与PCNA的表达呈正相关。HIF-1α、VEGF可能在缓解或抑制腰椎间盘退变中起到一定作用。
[Abstract]:Aim: to study the expression of hypoxia inducible factor-1 伪 (HIF-1 伪), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) in the nucleus pulposus of lumbar intervertebral disc of intact and ruptured fibrous rings, and to explore the correlation of these three cytokines. Methods: 42 specimens of degenerative nucleus pulposus of lumbar intervertebral disc were extirpated by middle and posterior lumbar dorsal surgery, including 21 cases of intact fibrous ring of nucleus pulposus of human lumbar intervertebral disc. Rupture of fibrous ring in nucleus pulposus of human lumbar intervertebral disc. The degeneration of nucleus pulposus of lumbar intervertebral disc was observed by HE staining under light microscope. Immunohistochemical SP method was used to detect the expression of hypoxia inducible factor-1 伪 (HIF-1 伪), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) in the nucleus pulposus of intact and ruptured fibrous rings. Results the density of chondrocytes in the intact medullary nucleus of the fibrous ring was significantly higher than that in the ruptured fibrous ring P0.01a, hypoxia inducible factor 1 伪 (HIF-1 伪), vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA A) in the intact fibrous annular nucleus pulposus. There was expression in the ruptured nucleus pulposus of the fibrous ring. The expression rate of three cytokines in the nucleus pulposus of the ruptured lumbar disc was significantly higher than that in the intact type of the fibrous ring P0.01. VEGF and HIF-1 伪, and the expression of VEGF and PCNA in the degenerative nucleus pulposus of the lumbar intervertebral disc was highly correlated with the expression rate of these cytokines in the nucleus pulposus of the degenerative lumbar intervertebral disc. Conclusion: the degeneration of lumbar intervertebral disc is accompanied by the decrease of cell density, and the degree of degeneration of the ruptured lumbar intervertebral disc is more serious than that of the intact fibrous ring. The expression rate of hypoxia inducible factor-1 伪 (HIF-1 伪), vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) was increased with the degeneration of lumbar intervertebral disc. There was a positive correlation between HIF-1 伪 and VEGF and PCNA in degenerative nucleus pulposus of lumbar intervertebral disc. HIF-1 伪 may play a role in relieving or inhibiting degeneration of lumbar intervertebral disc.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R681.53

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