自体脂肪源性干细胞联合无菌生物护创膜对慢性创面愈合的影响

发布时间：2018-05-20 20:41

本文选题：慢性创面 + 干细胞　；参考：《解放军医学杂志》2016年12期

【摘要】：目的评估自体脂肪源性干细胞(ADSCs)联合无菌生物护创膜对慢性创面愈合的作用。方法选取武警总医院收治的60例慢性创面患者,随机分为凡士林油纱组(A组)、无菌生物护创膜组(B组)、ADSCs联合无菌生物护创膜组(C组),每组20例。观察3组创面愈合情况、愈合时间及治疗后7、21、40d时的创面愈合率,治疗前、治疗7d、治疗14d时创面上皮基底层细胞增殖情况及治疗后50d创面上皮化情况,计数治疗前和治疗后7d创面上皮基底层新生微小血管密度(MVD)。结果与A、B组创面愈合情况比较,C组创周炎性反应轻,肉芽组织形成好,创缘上皮爬行速度快,且B组创面愈合情况好于A组。创面愈合时间A组B组C组,组间比较差异有统计学意义(P0.05)。3组治疗后各时间点创面愈合率、创面上皮基底层增殖细胞数、新生微小血管密度比较均为C组优于B组优于A组,差异有统计学意义(P0.05),治疗后50d创面上皮化情况亦为C组优于B组优于A组。结论自体ADSCs联合无菌生物护创膜可明显改善慢性创面修复细胞的增殖状态,促进创面血管再生,改善创面局部生长环境,加速创面愈合。
[Abstract]:Objective to evaluate the effect of autogenous adipose-derived stem cells (ADSCs) combined with aseptic biofilm on chronic wound healing. Methods Sixty patients with chronic wound were randomly divided into two groups: group A (group A) and group B (group B), with 20 cases in each group. The healing time, healing time and wound healing rate of the 3 groups were observed. The proliferation of epithelial basal layer cells was observed at 7 days before treatment, 14 days after treatment and 50 days after treatment. The neovascularization density of epithelial basal layer was counted before and 7 days after treatment. Results compared with group A, the wound healing in group C was less inflammatory than that in group A, the granulation tissue formed well and the wound edge epithelium crawled faster, and the wound healing in group B was better than that in group A. The healing time of wound in group A, group B, and group C, the difference between the two groups was statistically significant. The rate of wound healing, the number of proliferative cells of epithelial basal layer and the density of neovascularization in group C were better than those in group B at different time points after treatment (P 0.05.3), and the density of neovascularization in group C was better than that in group A. The difference was statistically significant (P 0.05), and the epithelialization of wounds in group C was better than that in group B 50 days after treatment. Conclusion autogenous ADSCs combined with aseptic biological wound protective membrane can obviously improve the proliferation of chronic wound healing cells, promote wound vascular regeneration, improve the local growth environment of wound, and accelerate wound healing.
【作者单位】：武警总医院再生医学科;
【分类号】：R641

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