脂肪来源干细胞（ADSCs）对光老化HaCaT细胞的实验研究

发布时间：2018-05-21 16:35

本文选题：ADSCs + 光老化　；参考：《青岛大学》2015年硕士论文

【摘要】：目的:通过体外共同培养ADSCs(Adipose stem cells,ADSCs)与Ha Ca T细胞,制造光老化模型,观察Ha Ca T细胞形态学变化,检测细胞培养液中抗氧化酶的活性,探讨脂肪来源干细胞(ADSCs)对光老化Ha Ca T细胞的抗老化作用。方法:设计实验组为ADSCs与Ha Ca T共培养组,培养4 8h后,给予紫外线UVA30 m J/c m照射24小时后,继续培养24h,对照组为单纯培养Ha Ca T,紫外线照射同实验组。观察Ha Ca T细胞形态学变化,检测两组细胞培养液中乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)活力。结果:对照组Ha Ca T细胞细胞数较少,细胞贴壁性较低,细胞间隙增大,细胞变圆或不规则,细胞体积变小。实验组较对照组细胞形态较好,圆形、不规则形、悬浮细胞较少。实验组SOD、LDH活力明显高于对照组(P0.05),实验组MDA含量明显低于对照组。结论:研究结果表明脂肪来源干细胞(ADSCs)对光老化有治疗作用,能够减轻UVA对Ha Ca T细胞的损伤,从而为抗光老化提供新的手段。
[Abstract]:Objective: to establish a photoaging model by co-culture of ADSCs(Adipose stem cells and Ha Ca T cells in vitro, observe the morphological changes of Ha Ca T cells, and detect the activities of antioxidant enzymes in the culture medium. To investigate the anti-aging effect of adipose derived stem cells (ADSCs) on photoaged Ha Ca T cells. Methods: the experimental group was designed as ADSCs and Ha Ca T co-culture group. After 48 hours of culture, ultraviolet UVA30 m J / cm was irradiated for 24 hours, and then cultured for 24 hours. The control group was treated with Ha Ca T, UV irradiation alone. The morphological changes of Ha Ca T cells were observed, and the activities of lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) in the culture medium of the two groups were detected. Results: in the control group, the number of Ha Ca T cells was less, the adhesion of cells was lower, the intercellular space was enlarged, the cells became round or irregular, and the cell volume became smaller. The shape of cells in the experimental group was better than that in the control group. The activity of MDA in the experimental group was significantly higher than that in the control group (P 0.05), and the content of MDA in the experimental group was significantly lower than that in the control group. Conclusion: the results suggest that ADSCs can be used to treat photoaging and reduce the damage of Ha Ca T cells induced by UVA, thus providing a new way to resist photoaging.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R622

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