芍药苷对大鼠脊髓损伤的神经保护机制

发布时间：2018-05-24 07:53

本文选题：芍药苷 + 脊髓损伤　；参考：《免疫学杂志》2016年05期

【摘要】：目的研究芍药苷对大鼠脊髓损伤(SCI)的神经保护机制。方法将大鼠随机分成对照组(假手术)、模型组(Allen’s打击法制备SCI模型)、治疗组1(术后每天腹腔注射芍药苷30 mg/kg)、治疗组2(芍药苷60 mg/kg),每组16只。手术当天为第1天,分别在第0、1、4、7、14天时采用BBB评分法对大鼠后肢运动功能进行测试。第4天和第14天每组分别处死8只大鼠,q PCR检测受损脊髓中TLR4和NF-κB p65 m RNA的表达;酶联免疫吸附实验测定检测受损脊髓中白细胞介素1β(IL-1β)、IL-6和肿瘤坏死因子α(TNF-α)的表达;Western blot检测TLR4、NF-κB p65、诱导型一氧化氮合酶(i NOS)、环氧酶2(COX-2)、Bcl-2和Caspase-3蛋白的表达。结果与对照组相比,模型组BBB评分明显降低(P0.05);与模型组相比,治疗组评分升高(P0.05),且治疗组2中评分较高。在第4天时,与对照组相比,模型组中TLR4、NF-κB p65、IL-1β、IL-6、TNF-α、i NOS、COX-2和Caspase-3的表达均增加(P0.05),Bcl-2的表达降低(P0.05);与模型组相比,治疗组中TLR4、NF-κB p65、IL-1β、IL-6、TNF-α、i NOS、COX-2和Caspase-3的表达均降低(P0.05),Bcl-2的表达升高(P0.05),这些变化均呈现剂量依赖性。第14天时,IL-1β、i NOS、COX-2、Caspase-3和Bcl-2的变化趋势与第4天时相同,但是各组中TLR4、NF-κB p65、IL-6和TNF-α表达均变化不大。结论芍药苷在大鼠脊髓损伤中的神经保护功能可能与TLR4/NF-κB信号通路介导的抗炎和抗凋亡作用相关。
[Abstract]:Objective to study the neuroprotective mechanism of paeoniflorin (paeoniflorin) on spinal cord injury (SCI) in rats. Methods rats were randomly divided into control group (sham operation) and model group (Allen 's strike method to prepare SCI model). The treatment group was 1 (paeoniflorin 30 mg/kg every day after operation) and 2 (paeoniflorin 60 mg/kg) in the treatment group, 16 in each group. The operation day was first days, respectively, in 0,1,4, BBB score was used to test the motor function of hind limbs of rats at 7,14 days. 8 rats were killed in each group on fourth days and fourteenth days. The expression of TLR4 and NF- kappa B p65 m RNA in the damaged spinal cord was detected by Q PCR; enzyme linked immunosorbent assay was used to detect the expression of interleukin 1 beta (IL-1 beta), IL-6 and tumor necrosis factor alpha in the damaged spinal cord. Stern blot detected TLR4, NF- kappa B p65, inducible nitric oxide synthase (I NOS), the expression of epoxide 2 (COX-2), Bcl-2 and Caspase-3 protein. Compared with the control group, the BBB score in the model group was significantly lower than that in the model group, and the treatment group was higher in score 2. At the fourth day, the model was compared with the control group. The expression of TLR4, NF- kappa B p65, IL-1 beta, IL-6, TNF- a, I NOS, COX-2 and Caspase-3 increased (P0.05). At fourteenth days, the change trend of IL-1 beta, I NOS, COX-2, Caspase-3 and Bcl-2 was the same as that at fourth days, but the TLR4, NF- kappa B p65, IL-6 and TNF- alpha were not changed much in each group. Conclusion the neuroprotective function of paeoniflorin in the spinal cord injury of rats may be related to the anti-inflammatory and anti apoptosis effect mediated by the signal pathway.
【作者单位】：河南省中医院(河南中医药大学第二附属医院)骨科脊柱病区;
【分类号】：R651.2

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