牵张成骨模型兔的建立与评估

发布时间：2018-05-26 01:16

本文选题：组织工程 + 模型　；参考：《中国组织工程研究》2017年16期

【摘要】：背景:牵张成骨技术在临床上成为重要的治疗大段骨缺损、骨肿瘤、骨髓炎等的有效方法,但在国内外进行牵张成骨的基础研究中,尚缺乏建立标准的牵张成骨模型。目的:建立兔股骨牵张成骨模型,评估兔股骨牵张成骨模型的成骨效果。方法:取20只雄性新西兰兔,采用新型的兔股骨牵张成骨器,建立兔股骨牵张成骨动物模型并通过标本一般形态和X射线片观察牵张成骨后的成骨效果。结果与结论:(1)标本一般形态:固定第14天,牵张间隙内新生骨组织颜色变浅,较前致密、匀称,呈圆柱状贯通骨折两端,与正常骨两端界限较前模糊;固定第35天,牵张间隙内可见新生骨组织表面与正常骨颜色、纹理相同,肉眼较难以分清正常骨组织和牵张间隙新生骨区的界限,骨密度明显增高。(2)X射线片检测结果:固定第14天,牵张支架固定稳定,骨折两端对线可,牵张间隙内可见连接正常骨两端的云雾状阴影,密度进一步增高;固定第35天,牵张支架固定稳定,骨折两端对线可,新生骨组织钙化完全,骨皮质完全形成,髓腔畅通;(3)结果证实:实验将自行研制的单臂式牵张支架应用于兔股骨牵张成骨实验,通过设计合理的建模步骤及规范的术后牵张成骨操作,成功建立了兔股骨牵张成骨模型。
[Abstract]:Background: distraction osteogenesis has become an important clinical treatment for large bone defects, bone tumors, osteomyelitis and other effective methods, but in the basic research of distraction osteogenesis at home and abroad, there is still a lack of standard distraction osteogenesis model. Objective: to establish a rabbit model of femoral distraction osteogenesis and evaluate the osteogenic effect of rabbit femoral distraction osteogenesis model. Methods: the animal model of distraction osteogenesis of rabbit femur was established by using a new type of rabbit distraction osteogenesis apparatus in 20 male New Zealand rabbits. The osteogenic effect of distraction osteogenesis was observed by the general morphology of the specimen and X-ray film. Results and conclusion: on the 14th day of fixation, the new bone tissue in the distraction space became lighter in color, denser and more symmetrical, with a cylindrical perforation between the two ends of the fracture, and the boundary of the two ends of the normal bone was more blurred than that of the normal bone, and on the 35th day of fixation, the new bone tissue in the distraction space became lighter, denser and more symmetrical. In the space of distraction, the surface of the new bone was the same as the normal bone, and the texture was the same. It was difficult for the naked eye to distinguish the boundary between the normal bone tissue and the new bone area of the distraction space. The results of X-ray examination showed that the bone density of the new bone tissue was significantly higher than that of the normal bone tissue on the 14th day of fixation. The distraction stent fixation is stable, the fracture ends can be aligned, the cloud shadow connecting the two ends of the normal bone can be seen in the distraction space, and the density is further increased; on the 35th day of fixation, the distraction stent fixation is stable, and the fracture ends can be aligned with each other. The results of complete calcification of new bone tissue, complete formation of bone cortex and unblocked medullary cavity confirmed that the self-made single-arm distraction scaffold was used in rabbit femur distraction osteogenesis. The rabbit femoral distraction osteogenesis model was successfully established by designing reasonable modeling steps and standardized distraction osteogenesis after operation.
【作者单位】：佛山健翔医院;广州市脑科医院;临沂市人民医院;
【基金】：2014山东省中青年科学家科研奖励基金(BS2014YY026) 2017佛山市卫生和计生局医学科研课题(20170161)~~
【分类号】：R-332;R68

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