异氟醚吸入麻醉对大鼠海马脑源性神经营养因子与Bcl-2表达的影响

发布时间：2018-05-27 17:01

本文选题：异氟醚 + 海马　；参考：《重庆医学》2017年29期

【摘要】：目的观察异氟醚(ISO)吸入麻醉对大鼠海马脑源性神经营养因子(BDNF)及B细胞淋巴瘤/白血病-2(Bcl-2)分子表达的影响。方法将54只SD大鼠分为对照组(n=6)、氧气(O2)组(n=24)和ISO组(n=24),对照组直接处死留取标本,O2组给予吸入40%O2和空气混合气体1h,ISO组以3%ISO诱导麻醉并以1.8%ISO维持1h。分别于麻醉前、诱导后、麻醉后30min、麻醉后60min、苏醒即刻观察大鼠呼吸频率及经皮血氧饱和度(SpO2)。O2组和ISO组分别于处理后6、12、24、72h处死大鼠取海马,采用半定量反转录PCR(RT-PCR)检测海马BDNF mRNA、Bcl-2mRNA的表达。结果大鼠呼吸频率在诱导后及麻醉后30、60min较麻醉前均有不同程度降低(P0.05);SpO2在诱导后、麻醉后30、60min及苏醒即刻与麻醉前比较,差异无统计学意义(P0.05)。ISO组大鼠海马BDNF mRNA表达水平与O2组比较在麻醉后12、24h降低(P0.05);麻醉后ISO组大鼠海马BDNF mRNA表达水平随时间呈下降趋势,在12、24h明显低于对照组(P0.05)。ISO组大鼠海马Bcl-2mRNA表达水平在所有时间点均低于对照组与O2组(P0.05)。结论 ISO麻醉可对大鼠海马BDNF及Bcl-2的表达产生抑制作用。
[Abstract]:Objective to investigate the effects of isoflurane (ISO) inhalation anesthesia on the expression of brain-derived neurotrophic factor (BDNF) and B-cell lymphoma / leukemic Bcl-2) molecules in the hippocampus of rats. Methods Fifty-four Sprague-Dawley rats were divided into two groups: the control group (n = 6) and the ISO group (n = 24). The control group was killed and the control group was killed directly. The rats in the control group received 3%ISO induction anesthesia and maintained the anesthesia with 1.8%ISO for 1 h after inhaling 40%O2 and air mixture gas for 1 h. Before and after induction, 30 minutes after anesthesia and 60 minutes after anesthesia, the respiratory rate and percutaneous oxygen saturation (SPO _ 2 路O _ 2) of the rats were observed immediately after anesthesia, and the rats in the ISO group were killed to take out the hippocampus at 2 472 h after treatment. The expression of BDNF mRNAs Bcl-2 mRNA in hippocampus was detected by semi-quantitative reverse transcription PCR- RT-PCR- PCR. Results after induction and 30 minutes after anesthesia, the respiratory rate of rats was decreased in varying degrees compared with that before anesthesia. After induction, 30 minutes after anesthesia, 60 minutes after anesthesia and immediately after anesthesia, there was a comparison between the rate of respiration and that before anesthesia. There was no significant difference in the expression of BDNF mRNA between the two groups. The expression of BDNF mRNA in hippocampus of rats in the ISO group decreased with time compared with that in the O _ 2 group at 1224 h after anesthesia, and the expression of BDNF mRNA in the hippocampus of the rats in the ISO group decreased with the time after anesthesia, and the expression of BDNF mRNA in the hippocampus of the rats in the ISO group decreased with time. The expression of Bcl-2mRNA in hippocampus of rats in the control group was significantly lower than that in the control group and O2 group at 24 h after 12h. The expression of Bcl-2mRNA in hippocampus was lower than that in the control group and O2 group at all time points. Conclusion ISO anesthesia can inhibit the expression of BDNF and Bcl-2 in hippocampus of rats.
【作者单位】：遵义医学院附属医院麻醉科;贵州省麻醉与器官保护基础研究重点实验室;
【基金】：贵州省科学技术基金资助项目(黔科合J字LKZ[2011]29)
【分类号】：R614

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