曲美他嗪预先给药对异丙酚致大鼠心肌损伤的影响

发布时间：2018-06-01 21:51

本文选题：曲美他嗪 + 二异丙酚　；参考：《兰州大学》2015年硕士论文

【摘要】：目的：评价曲美他嗪预先给药对异丙酚致大鼠心肌损伤的影响。方法：健康SD大鼠40只,2.5月龄,体重240-250 g,采用随机数字表法,将其分为4组(n＝10)：脂肪乳组(L组)静脉输注10%脂肪乳12 h,输注速率30 mg·kg-1·h-1;异丙酚组(P组)静脉输注1%异丙酚中/长链脂肪乳注射液12h,输注速率30 mg·kg-1·h-1;不同剂量曲美他嗪组(TL组和TH组)分别静脉注射曲美他嗪2mg/kg和5mg/kg,1.5 h后静脉输注异丙酚12 h,输注速率30 mg·kg-1·h-1。于给药开始后6、12 h时采集颈内静脉血样1.5 ml检测肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、α-羟丁酸脱氢酶(a-HBDH)、肌钙蛋白(cTnI)、甘油三 (TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平。给药开始后12 h采血后取心肌组织于光镜及电镜下观察心肌病理学结果。结果：与L组比较,P组和TL组血清CK、CK-MB、LDH、a-HBDH活性和cTnI、TG浓度升高,HDL浓度降低(P0.01),LDL浓度差异无统计学意义(P0.05),TH组血清TG, HDL和LDL浓度升高(P0.01),其余指标差异无统计学意义(P0.05)。与P组比较,TL组和TH组血清CK、CK-MB、LDH、 a-HBDH活性和cTnI浓度降低,TL组血清TG浓度升高,TH组血清TG、HDL和LDL浓度升高(P0.01)。与TL组比较,TH组血清CK、CK-MB、LDH、a-HBDH活性和cTnI浓度降低,TG、HDL、LDL浓度升高(P0.01)。心肌在光镜及电镜下可见线粒体、肌纤维和Z线损伤,L组未观察到。结论：曲美他嗪预先给药可减轻异丙酚致心肌损伤,但会引起血脂升高。
[Abstract]:Aim: to evaluate the effect of trimetazidine on myocardial injury induced by propofol in rats. Methods: 40 healthy SD rats (2.5 months old, weight 240-250 g) were randomly selected. It was divided into 4 groups: fat milk group (L group): 10% fat milk infusion for 12 h, infusion rate 30 mg kg-1 h-1; propofol group (P group) 1% propofol medium / long chain fat emulsion injection 12 h, infusion rate 30 mg kg-1 h-1; different doses trimetazidine Trimetazidine (2mg/kg) and propofol (5 mg / kg) were injected intravenously for 12 h and 30 mg kg-1 h-1, respectively. The levels of creatine kinase (CK), creatine kinase isoenzyme (CK-MBN), lactate dehydrogenase (LDH), 伪 -hydroxybutyrate dehydrogenase (伪 -hydroxybutyrate dehydrogenase), cTnI, triglyceride (TGG), low density lipoprotein (LDL) and high density lipoprotein (HDL) were measured 6 h after administration. Myocardial tissue was collected 12 hours after administration and the pathological results of myocardium were observed under light microscope and electron microscope. Results: compared with L group, there was no significant difference between P group and TL group in serum CKCK-MBN LDHna-HBDH activity and cTnIttriglyceride concentration. There was no significant difference in serum TGG, HDL and LDL concentration in serum of P0.05TH group, and there was no significant difference in other indexes. Compared with P group, serum CK CK-MBN LDH, a-HBDH activity and cTnI concentration were decreased in TL group and th group. Serum TG level in TL group was higher than that in th group. Serum TGP HDL and LDL concentration in th group were higher than that in th group (P 0.01). Compared with TL group, the activity of serum CKK-MBN LDHa-HBDH and the concentration of cTnI decreased in th group. Myocardial mitochondria, muscle fiber and Z line injury were not observed in group L under light microscope and electron microscope. Conclusion: trimetazidine can reduce myocardial injury induced by propofol, but it can cause hyperlipidemia.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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