七氟醚单次和多次暴露对新生鼠海马神经元结构的影响

发布时间：2018-06-03 22:35

本文选题：七氟醚 + 新生大鼠　；参考：《临床麻醉学杂志》2017年04期

【摘要】：目的探讨等同时间不同次数七氟醚暴露对新生大鼠海马CA1细胞形态及超微结构的影响。方法出生7d的SD雄性大鼠45只,体重14~18g,随机均分为三组:对照组(C组)、单次七氟醚暴露组(SS组)和多次七氟醚暴露组(TS组)。SS组于出生后第7天吸入1次3%七氟醚6h;TS组于出生后第7、8、9天每天吸入1次3%七氟醚2h,累计6h;C组在相应日龄吸入60%氧气。三组于出生后第14天灌注取脑,采用HE和尼氏染色观察大鼠海马CA1区锥体神经元形态及数量变化;同时,透射电镜下观察该区域神经元超微结构及突触后致密物厚度和活性区长度。结果 HE和尼氏染色显示:与C组比较,SS组和TS组海马CA1细胞排列稀疏,神经元数量明显减少(P0.05);与SS组比较,TS组海马CA1神经元数量明显减少(P0.05)。电镜结果显示:与C组比较,SS组和TS组海马CA1神经元亚细胞器均有不同程度的受损,突触后致密物厚度明显变薄,活性区长度明显缩短(P0.05);与SS组比较,TS组神经元亚细胞器损伤更明显,突触后致密物厚度更薄,活性区长度缩短更严重(P0.05)。结论七氟醚单次和多次暴露均会引起新生大鼠海马CA1区锥体神经元数量减少及细胞超微结构的改变,等同时间多次暴露比单次暴露对神经元形态的损伤更严重。
[Abstract]:Objective to investigate the effects of different times of sevoflurane exposure on the morphology and ultrastructure of hippocampal CA1 cells in neonatal rats. Methods Forty-five Sprague-Dawley male rats were born at 7 days. Weight 14g / 18g, randomly divided into three groups: control group (C), single sevoflurane exposure group (SS group) and multiple sevoflurane exposure group (TS group). The SS group inhaled 3% sevoflurane once a day on the 7th day after birth. The TS group was inhaled 1 / day on the 7th day after birth. 3% sevoflurane for 2 h and 6 h cumulative for group C inhaled 60% oxygen at the corresponding age. The rats in the three groups were perfused on the 14th day after birth to observe the morphology and number of pyramidal neurons in the hippocampal CA1 region by HE and Nissl staining. The ultrastructure of neurons, the thickness of postsynaptic dense substance and the length of active area were observed under transmission electron microscope. Results HE and Nissl staining showed that compared with group C, CA1 cells in hippocampus of SS group and TS group were sparsely arranged and the number of neurons decreased significantly (P 0.05), and the number of CA1 neurons in hippocampus of TS group was significantly decreased compared with that of SS group (P 0.05). The results of electron microscope showed that the suborganelles of hippocampal CA1 neurons in SS group and TS group were damaged to some extent, and the thickness of postsynaptic dense substance became thinner than that in C group. Compared with SS group, the suborganelle damage of neurons in TS group was more obvious, the thickness of postsynaptic dense substance was thinner, and the length of reactive area was shorter than that of SS group. Conclusion both single and multiple exposures of sevoflurane can decrease the number of pyramidal neurons and change the ultrastructure of hippocampal CA1 in neonatal rats. Multiple exposures of sevoflurane at the same time are more serious than single exposures to the morphology of neurons.
【作者单位】：宁夏医科大学总医院麻醉科;
【基金】：宁夏自然科学基金资助(NZ15135)
【分类号】：R614

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