沉默信息调节因子2在人骨关节炎软骨组织及细胞中的表达

发布时间：2018-06-05 10:44

本文选题：骨关节炎 + 软骨组织　；参考：《安徽医科大学学报》2017年08期

【摘要】：目的检测沉默信息调节因子2(SIRT2)在人软骨中的表达,探讨其在骨关节炎(OA)与正常软骨组织及细胞中表达的差异性。方法选取临床20例骨关节炎关节软骨,为OA组;另取10例正常关节软骨,为对照组。免疫荧光染色法检测SIRT2在两组软骨组织的表达分布及表达情况。分离软骨细胞并进行细胞传代培养,甲苯胺蓝染色进行细胞鉴定,Western blot法检测软骨组织及细胞中SIRT2的表达情况。结果免疫荧光染色结果显示SIRT2表达于软骨组织中,分布在细胞核与细胞质中,OA组SIRT2阳性细胞率较对照组明显降低(P0.01);Western blot结果显示OA组软骨组织中SIRT2表达显著低于对照组(P0.01),软骨细胞中SIRT2的表达亦显著低于对照组(P0.01)。结论 SIRT2在人类软骨组织中有表达,具体表达在细胞核与细胞质中,SIRT2表达降低可能导致骨关节炎的发生及进展。
[Abstract]:Objective to detect the expression of silencing signaling factor 2sirt2 in human cartilage, and to explore the difference between the expression of silencing signal regulator 2SIRT2 in osteoarthritis (OAA) and normal cartilage tissues and cells. Methods Clinical 20 cases of osteoarthritis articular cartilage were selected as OA group and 10 cases of normal articular cartilage as control group. The distribution and expression of SIRT2 in cartilage tissue were detected by immunofluorescence staining. Chondrocytes were isolated and cultured. The expression of SIRT2 in chondrocytes and cells was detected by toluidine blue staining and Western blot assay. Results Immunofluorescence staining showed that SIRT2 was expressed in cartilage. The percentage of SIRT2 positive cells in OA group was significantly lower than that in control group. The results of Western blot showed that the expression of SIRT2 in cartilage tissue of OA group was significantly lower than that in control group (P 0.01), and the expression of SIRT2 in chondrocytes was significantly lower than that in control group (P 0.01). Conclusion SIRT2 is expressed in human chondrocytes, and the decrease of SIRT2 expression in nucleus and cytoplasm may lead to the occurrence and progression of osteoarthritis.
【作者单位】：安徽医科大学第四附属医院骨外科;安徽医科大学第一附属医院关节与骨肿瘤外科;
【基金】：国家自然科学基金(编号:81171173) 安徽医科大学校科学研究基金(编号:2015xkj042)
【分类号】：R684.3

【参考文献】