饮酒通过内质网应激促进小鼠乳腺癌恶性进展

发布时间：2018-06-06 19:30

本文选题：酒精 + 乳腺癌　；参考：《中国实验动物学报》2017年06期

【摘要】：目的通过动物实验及体外实验探索饮酒是否促进乳腺癌恶性进展,且这一作用是否与其诱导的慢性应激有关。方法利用小鼠乳腺癌移植瘤模型,体内观察2%乙醇慢性处理的小鼠乳腺癌E0771细胞对体内细胞生长转移的影响;利用体外细胞学实验观察0.2%乙醇慢性诱导对小鼠乳腺癌细胞E0771增殖、迁移及非锚定生长能力的影响;同时用分子生物学方法探索细胞内ROS水平及慢性内质网应激蛋白如p-e IF2a、Bip、XBP1-s等蛋白表达水平是否发生改变。结果与对照组相比,饮酒组小鼠体内肿瘤生长速度加快,转移增加;体外酒精处理可以显著促进乳腺癌细胞增殖、迁移等恶性生物学行为,酒精慢性处理可以诱导ROS、内质网应激活化蛋白p-e IF2a、Bip、XBP1-s表达上升。结论饮酒可能通过诱导乳腺癌细胞内质网应激促进其恶性进展。
[Abstract]:Objective to investigate whether alcohol consumption promotes the malignant progression of breast cancer and whether this effect is related to chronic stress induced by alcohol consumption in animal and in vitro experiments. Methods the effect of 2% ethanol on the growth and metastasis of mouse breast cancer E0771 cells was observed in vivo by using mouse breast cancer transplanted tumor model. The effects of 0.2% ethanol on the proliferation, migration and unanchored growth of mouse breast cancer cell line E0771 were observed by cytological experiments in vitro. At the same time, the cellular Ros level and the expression level of chronic endoplasmic reticulum stress proteins such as p-e IF2a BipBP1-s were investigated by molecular biology. Results compared with the control group, the tumor growth rate and metastasis increased in the drinking group, and the malignant biological behaviors such as proliferation and migration of breast cancer cells were significantly promoted by alcohol treatment in vitro. Chronic alcohol treatment induced an increase in the expression of endoplasmic reticulum stress activating protein p-e IF2aP1-s. Conclusion drinking may promote the malignant progression of breast cancer cells by inducing endoplasmic reticulum stress.
【作者单位】：安徽医科大学基础医学院病理生理学实验室;
【基金】：国家自然科学基金(No.30973424,No.81272258)
【分类号】：R737.9

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