miR-494下调Sox9的表达对骨肉瘤细胞MG-63增殖和侵袭的影响

发布时间：2018-06-07 15:51

本文选题：miR- + 骨肉瘤　；参考：《肿瘤防治研究》2017年02期

【摘要】：目的探讨miR-494对人骨肉瘤细胞MG-63增殖与侵袭的影响,并验证Sox9是否为miR-494的靶基因。方法过表达miR-494后,利用CCK-8、克隆形成实验检测MG-63细胞的增殖,利用Transwell实验检测细胞的侵袭能力。Western blot与荧光定量PCR检测Sox9蛋白与m RNA水平的表达。结果 MG-63细胞转染腺病毒miR-494后,miR-494表达明显升高(t=36.78,P=0.000),Ad-miR-494转染组MG-63细胞增殖(F=1.711,P=0.012)、克隆形成(F=2.742,P=0.019)和侵袭能力(F=1.653,P=0.006)较Ad-GFP组下降。过表达miR-494后,Sox9蛋白(F=5.827,P=0.021)和m RNA表达水平(F=5.827,P=0.021)下降。而Sox9的下调使MG-63细胞增殖(t=27.54,P=0.042)、克隆形成(t=29.64,P=0.026)和侵袭能力(t=32.48,P=0.016)下降。结论 miR-494通过Sox9抑制骨肉瘤细胞MG-63的增殖与侵袭。
[Abstract]:Objective to investigate the effect of miR-494 on the proliferation and invasion of human osteosarcoma cell line MG-63 and to verify whether Sox9 is a target gene of miR-494. Methods after overexpression of miR-494, CCK-8 was used to detect the proliferation of MG-63 cells, and Transwell assay was used to detect the invasion ability of MG-63 cells. Western blot and fluorescent quantitative PCR were used to detect the expression of Sox9 protein and m RNA. Results after MG-63 cells were transfected with adenovirus miR-494, the expression of miR-494 increased significantly, and the proliferation of MG-63 cells in Ad-miR-494 transfection group was significantly higher than that of Ad-GFP group. The cell proliferation of MG-63 cells was 1.711P0. 012, and the clone formation of MG-63 cells was decreased compared with that of Ad-GFP group (F1.653P0. 006). After overexpression of miR-494, the expression level of Sox9 protein FG 5.827 P0.021) and m RNA expression level (F5. 827 P0. 021) decreased. However, down-regulation of Sox9 decreased the proliferation of MG-63 cells (27.54%), clone formation (29. 64%) and invasion ability (32. 48%) of MG-63 cell line (0. 016). Conclusion miR-494 inhibits the proliferation and invasion of osteosarcoma cell line MG-63 through Sox9.
【作者单位】：西南医科大学附属医院检验科;
【基金】：2014年四川省科学技术厅科研专项基金(14JC0801) 2016年泸州市科技局项目科研专项基金(15193)
【分类号】：R738.1

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