舒芬太尼对小鼠周围神经损伤后脊髓星形胶质细胞活化的影响

发布时间：2018-06-08 20:00

本文选题：舒芬太尼 + 周围神经系统　；参考：《临床麻醉学杂志》2017年03期

【摘要】：目的探讨舒芬太尼对小鼠单侧坐骨神经损伤后脊髓星形胶质细胞活化的影响。方法清洁级健康雄性BALB/c小鼠80只,6~8周龄,体重18~22g。采用随机数字量表法分为四组:舒芬太尼高剂量组(H组)、舒芬太尼中剂量组(M组)、舒芬太尼低剂量组(L组)和模型组(MO组),每组20只。制备单侧坐骨神经离断损伤模型后,H、M、L组分别腹腔注射舒芬太尼10μg/kg、5μg/kg、2.5μg/kg,MO组腹腔注射等容量生理盐水,连续注射3d,每天1次。于4、8、12周检测小鼠坐骨神经功能指数(SFI)。于2、4、8、12周每组随机处死的5只小鼠,取其损伤同侧L4~L6脊髓节段。光学显微镜下观察病理学结果。采用免疫组化法检测脊髓各时点GFAP的表达。结果 H、M组SFI明显高于L、MO组(P0.05),L、MO组SFI差异无统计学意义;H、M组脊髓前角神经元形态较好,L、MO组脊髓前角神经元形态较差。与MO组比较,各时点H、M、L组GFAP的表达上调(P0.05);与L组比较,各时点H、M组GFAP的表达上调(P0.05)。结论舒芬太尼促进小鼠周围神经损伤后脊髓星形胶质细胞活化,利于周围神经损伤后的修复。
[Abstract]:Objective to investigate the effect of sufentanil on the activation of spinal astrocytes after unilateral sciatic nerve injury in mice. Methods 80 healthy male BALB / c BALB / c mice of clean grade were 8 weeks old and weighing 1822 g. Four groups were randomly divided into four groups: sufentanil high dose group (n = 20), sufentanil medium dose group (n = 20), sufentanil low dose group (n = 20) and model group (n = 20). After unilateral sciatic nerve injury was established, sufentanil (10 渭 g / kg 路kg ~ (5) 渭 g 路kg ~ (-1) and 2.5 渭 g / kg ~ (2. 5) 渭 g 路kg ~ (-1) MO group were injected intraperitoneally respectively with sufentanil once a day for 3 days. The sciatic nerve function index (SFI) of mice was measured at 12 weeks. At 12 weeks, 5 mice were randomly killed in each group, and the ipsilateral L4 and L6 spinal cord segments were taken. The pathological results were observed under optical microscope. The expression of GFAP in spinal cord was detected by immunohistochemical method. Results there was no significant difference in SFI between Ham group and Lemo group. The morphology of spinal cord anterior horn neurons in Ham M group was better than that in Lemo group. Compared with MO group, the expression of GFAP was upregulated at different time points in HGFAP group, and the expression of GFAP was upregulated in HfM group at different time points compared with that in L group. Conclusion Sufentanil can promote the activation of spinal astrocytes after peripheral nerve injury in mice, which is beneficial to the repair of peripheral nerve injury.
【作者单位】：内蒙古自治区赤峰市医院麻醉科;内蒙古自治区赤峰市医院骨科;
【分类号】：R614

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