大鼠心肾联合移植模型的建立

发布时间：2018-06-10 03:13

本文选题：肾移植 + 心脏移植　；参考：《实用医学杂志》2017年16期

【摘要】：目的探讨大鼠心肾联合移植模型手术方法。方法心肾联合移植组雄性近交系Lewis大鼠做为肾移植的供、受者,原位灌注取左肾及心脏,肾动脉重建用套袖吻合法,肾静脉用内撑法间断吻合,左肾原位移植后将供体心脏主动脉和肺动脉分别与受体腹主动脉和下腔静脉端侧吻合,受体右肾立即切除,以Lewis大鼠右肾切除为对照组。结果心肾热缺血时间10 s,供体取肾时间(30.0±3.0)min,供心摘取时间(6.0±1.8)min,冷缺血时间肾脏(40±3.2)min、心脏(70±4.1)min,受体手术时间(95±5.4)min,实施移植手术21例。手术成功率为80.9%,术后30 d肾功能及心率与对照组比较差异无统计学意义。结论建立大鼠心肾联合移植模型稳定可靠,可作为心肾联合移植的理想模型,可应用于多脏器移植免疫的基础研究。
[Abstract]:Objective to explore the operative method of combined heart and kidney transplantation in rats. Methods in the combined heart and kidney transplantation group, the male inbred Lewis rats were used as the donor of renal transplantation. The left kidney and heart were obtained by in-situ perfusion, the renal artery was reconstructed by sleeve anastomosis, and the renal vein was anastomosed intermittently by internal brace. After orthotopic transplantation of left kidney, donor aorta and pulmonary artery were anastomosed with recipient abdominal aorta and inferior vena cava respectively. The recipient right kidney was resected immediately and Lewis rat right nephrectomy was used as control group. Results the time of warm ischemia of heart and kidney was 10s, the time of donor kidney was 30.0 卤3.0 minutes, the time of donor heart extraction was 6.0 卤1.8 minutes, the time of cold ischemia was 40 卤3.2 minutes, the heart was 70 卤4.1 minutes, the operative time of recipient was 95 卤5.4 minutes. The successful rate of operation was 80.9. There was no significant difference in renal function and heart rate 30 days after operation between the control group and the control group. Conclusion the rat model of combined heart and kidney transplantation is stable and reliable. It can be used as an ideal model for combined heart and kidney transplantation and can be applied to the basic research of multiple organ transplantation immunity.
【作者单位】：广州中医药大学第二附属医院;
【基金】：国家自然科学基金资助(编号:30701074,81373523)
【分类号】：R-332;R617

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