移植人羊膜间充质干细胞促进脊髓损伤大鼠神经功能恢复

发布时间：2018-06-13 03:15

本文选题：间充质干细胞 + 羊膜　；参考：《中华神经创伤外科电子杂志》2016年06期

【摘要】：目的研究移植人羊膜间充质干细胞(hAMSCs)是否促进脊髓损伤大鼠神经功能恢复,探索其可能作用机制。方法 60只雌性SD大鼠按照随机数字表法分为磷酸盐缓冲液(PBS)治疗组(30只)和hAMSCs治疗组(30只)。脊髓损伤采用脊髓撞击损伤模型,hAMSCs或PBS立刻移植到离脊髓损伤中心2 mm的头尾两端。免疫荧光检测细胞分化,血管再生和轴突再生。酶联免疫吸附剂测定试剂盒检测脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)含量,BBB运动功能评分检测行为学。结果在脊髓损伤后14 d、21 d和28 d,hAMSCs治疗组BBB评分分别为(8.75±0.701)、(10.375±0.532)和(12.125±0.350),高于PBS组(6.0±0.463)、(7.25±0.412)和(9.125±0.440),差异具有统计学意义(P0.05)。在第7天和第14天,hAMSCs治疗组BDNF表达水平分别为(75.138±4.367)pg/mg和(66.483±4.099)pg/mg,高于PBS组(43.901±3.607)pg/mg和(41.108±3.848)pg/mg,差异具有统计学意义(P0.05)。在第7天,第14天和第28天,hAMSCs治疗组VEGF表达水平分别为(23.328±2.463)pg/mg,(22.301±2.223)pg/mg和(14.855±1.282)pg/mg,高于PBS组(9.978±1.572)pg/mg,(9.271±1.496)pg/mg和(7.113±1.123)pg/mg,差异具有统计学意义(P0.05)。hAMSCs治疗组血管数目(17.5±2.102)高于PBS组(6.25±1.750),差异具有统计学意义(P0.05)。hAMSCs治疗组小鼠抗5羟色胺阳性神经纤维面积(3486±203.643)和GAP43阳性神经纤维面积(4568.25±253.881)高于PBS组(2070.25±156.344)和(2455.725±314.475),差异具有统计学意义(P0.05)。结论移植hAMSCs能促进脊髓损伤大鼠神经功能恢复,其作用机制可能是通过增加神经营养因子表达,促进血管再生和轴突再生。因此hAMSCs移植是治疗脊髓损伤的理想方法。
[Abstract]:Objective to investigate whether the transplantation of human amniotic mesenchymal stem cells (hAMSCs) can promote the recovery of neural function in rats with spinal cord injury (sci) and explore its possible mechanism. Methods 60 female Sprague-Dawley rats were randomly divided into two groups: phosphate buffer (PBS) group (n = 30) and hAMSCs group (n = 30). HAMSCs or PBS were transplanted to the head and tail of spinal cord injury center 2 mm from the center of spinal cord injury. Cell differentiation, angiogenesis and axon regeneration were detected by immunofluorescence. The contents of BDNF and VEGF were measured by enzyme linked immunosorbent assay kit (Elisa) and BBB motor function score (BBB) were used to detect the behavior of brain derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF). Results the BBB scores in the treatment group were 8.75 卤0.701 卤0.532 and 12.125 卤0.350 on the 14th and 28th days after spinal cord injury, respectively, which were higher than those in the PBS group (6.0 卤0.463 卤0.412) and 9.125 卤0.4400.The difference was statistically significant. On the 7th and 14th day, the expression of BDNF in the treatment group was 75.138 卤4.367)pg/mg and 66.483 卤4.099 PG / mg, respectively, which was higher than that in the PBS group (43.901 卤3.607)pg/mg and 41.108 卤3.848 g / mg). The difference was statistically significant. On the seventh day, On the 14th day and 28th day, the expression of VEGF in the treatment group was 23.328 卤2.463pg / mg / 2.223)pg/mg and 14.855 卤1.282 / mg / mg, respectively, which was higher than that in the PBS group (9.978 卤1.572g / mg / mg) and 7.113 卤1.123g / mg / g, respectively. The difference was statistically significant (P < 0.05 卤2.102) and the number of blood vessels in the treatment group was significantly higher than that in the control group (6.25 卤1.750). The difference was statistically significant. The areas of tryptamine positive nerve fibers (3486 卤203.643) and GAP43 positive nerve fibers (4568.25 卤253.881) were higher than those of PBS (2070.25 卤156.344) and 2455.725 卤314.475m (P 0.05). Conclusion Transplantation of hAMSCs can promote the recovery of nerve function in rats with spinal cord injury, and its mechanism may be to promote vascular regeneration and axonal regeneration by increasing the expression of neurotrophic factor. Therefore, hAMSCs transplantation is an ideal method for the treatment of spinal cord injury.
【作者单位】：解放军陆军总医院附属八一脑科医院;解放军陆军总医院妇产科;河北医科大学第二医院神经外科;
【基金】：全军医药科技“十二五”重点项目(BWS11J002);全军医药科技“十二五”重点项目(BWS12J010) 国家自然科学基金(81401032)
【分类号】：R651.2

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