半月板损伤并发膝关节滑膜炎患者局部滑膜组织及滑液中基质金属蛋白酶3、9、13的表达观察

发布时间：2018-06-15 04:36

  本文选题：滑膜炎 + 半月板损伤　； 参考：《山东医药》2017年19期

【摘要】：目的观察半月板损伤导致的滑膜炎患者滑膜滑液中基质金属蛋白酶3(MMP-3)、基质金属蛋白酶9(MMP-9)和基质金属蛋白酶13(MMP-13)的表达变化。方法 128例半月板损伤患者,根据是否有伴有滑膜炎分为半月板损伤诱发滑膜炎者81例(观察组)和单纯半月板损伤者47例(对照组)。分别采用荧光定量PCR法和免疫组化法检测两组滑膜组织中MMP-3、MMP-9和MMP-13 mRNA和蛋白,采用ELISA法检测两组滑液MMP-3、MMP-9和MMP-13。结果观察组滑膜组织中MMP-3、MMP-9和MMP-13 mRNA相对表达量分别为3.12±0.41、2.10±0.24、3.01±0.30,对照组分别为1.53±0.35、0.87±0.18、1.12±0.21,P均0.05;观察组滑膜组织中MMP-3、MMP-9和MMP-13蛋白相对表达量分别为394.1±20.6、170.5±14.8、190.7±13.6,对照组分别为204.5±15.3、90.3±8.4、109.3±10.4,P均0.05。观察组滑液组织中MMP-3、MMP-9和MMP-13 mRNA相对表达量分别为12.5±2.1、15.7±2.4、9.5±1.9,对照组分别为19.4±2.6、17.5±2.3、14.7±2.4,P均0.05。观察组Ayral滑膜炎评分≤60分者滑液MMP-3、MMP-9和MMP-13水平明显低于Ayral滑膜炎评分60分者(P0.05)。结论半月板损伤导致的滑膜炎患者滑膜、滑液存在MMP-3、MMP-9和MMP-13高表达。MMP-3、MMP-9和MMP-13的异常表达可能与半月板损伤滑膜炎的发生有关。
[Abstract]:Objective to observe the expression of matrix metalloproteinase 3 (MMP-3), matrix metalloproteinase 9 (MMP-9) and matrix metalloproteinase 13 (MMP 13) in synovial fluid of patients with synovitis caused by meniscus injury. Methods 128 patients with meniscus injury were divided into two groups according to whether there was synovitis induced by meniscus injury in 81 cases (observation group) and 47 cases in simple meniscus injury (control group). The mRNA and protein of MMP-3, MMP-9 and MMP-13 in synovial tissue of two groups were detected by fluorescence quantitative PCR and immunohistochemistry, respectively, and MMP-3, MMP-9 and MMP-13 in synovial fluid of two groups were detected by Elisa. Results the relative expressions of MMP-3, MMP-9 and MMP-13 mRNA in synovial tissue of observation group were 3.12 卤0.41 卤0.24 卤3.01 卤0.30, respectively, and those in control group were 1.53 卤0.35 卤0.18 卤0.21 卤1.12 卤0.21, respectively, and the relative expression of MMP-3MMP-9 and MMP-13 protein in synovial tissue of observation group were 394.1 卤20.6170.5 卤14.8190.7 卤13.6and 204.5 卤15.30.93 卤8.4109.3 卤10.4P respectively (P < 0.05). The relative expression of MMP-3MMP-9 and MMP-13 mRNA in synovial tissue of observation group was 394.1 卤20.6170.5 卤14.8190.7 卤13.60.The relative expression of MMP-3MMP-9 and MMP-13 protein in synovial tissue of observation group was 394.1 卤20.6170.5 卤14.8190.7 卤13.6, respectively. The relative expressions of MMP-3, MMP-9 and MMP-13 mRNA in synovial fluid were 12.5 卤2.1 卤2.4 卤9.5 卤1.9 in the observation group and 19.4 卤2.6 卤17.5 卤2.4 卤2.4 in the control group, respectively. In the observation group, the levels of MMP-9 and MMP-13 in synovial fluid with Ayral synovitis score 鈮，

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