丙酮酸乙酯对脾切除术后老龄大鼠认知功能的影响

发布时间：2018-06-16 11:54

本文选题：丙酮酸乙酯 + 术后认知功能障碍　；参考：《临床麻醉学杂志》2017年07期

【摘要】：目的探讨丙酮酸乙酯(ethyl pyruvate,EP)对脾切除术后老龄大鼠认知功能的影响。方法健康雄性SD大鼠84只,18月龄,体重500~600g,采用随机数字表法将其分为四组,每组21只:对照组(C组)、手术组(S组)、EP溶液组(E组)和溶剂组(R组)。S组、E组和R组行脾切除术,E组腹腔注射EP 40mg/kg,R组腹腔注射等量EP溶液的溶剂,S组和C组腹腔注射等量生理盐水。于术前5d、术后1、3、7d进行Morris水迷宫实验,记录大鼠逃避潜伏期和游泳距离,水迷宫实验结束后处死大鼠,采用Western blot和RT-PCR法检测四组大鼠海马高迁移率族蛋白1(high mobility group box 1,HMGB1)及晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)的蛋白含量和mRNA表达量。结果与C组比较,术后1、3dS组、E组和R组逃避潜伏期和游泳距离明显延长,海马HMGB1、RAGE蛋白含量和mRNA表达量明显增加(P0.05)。与S组比较,术后1、3dE组逃避潜伏期和游泳距离明显缩短,海马HMGB1、RAGE蛋白含量和mRNA表达量明显减少(P0.05)。与术前比较,术后1、3dS组、E组和R组逃避潜伏期和游泳距离明显延长(P0.05)。结论丙酮酸乙酯可能通过下调海马HMGB1及RAGE的表达改善认知功能。
[Abstract]:Objective to investigate the effect of ethyl pyruvate EPV on cognitive function of aged rats after splenectomy. Methods 84 male Sprague-Dawley rats were randomly divided into four groups. 21 rats in each group: control group (group C), operation group (group S), group E (group E) and solvent group (group R, group C). Group S, group E, and group R, respectively, were treated with splenectomy. Group E was injected intraperitoneally with EP of 40 mg / kg 路kg ~ (-1) / L, group S and group C were injected intraperitoneally with the same amount of EP solution, group S and group C were injected intraperitoneally with the same amount of EP solution. Shoot the same amount of saline. Morris water maze test was performed 5 days before operation and 3 days after operation. The escape latency and swimming distance of the rats were recorded. The rats were killed after the water maze test. Western blot and RT-PCR were used to detect the protein content and mRNA expression of high mobility group protein 1(high mobility group box 1 (HMGB1) and advanced glycosylation end product receptor for advanced glycation end products rages in the hippocampus of rats in the four groups. Results compared with group C, the escape latency and swimming distance of group E and group R were significantly prolonged, and the protein content and mRNA expression of HMGB1 + rage in hippocampus increased significantly (P 0.05). Compared with group S, the escape latency and swimming distance in group E were significantly shortened, and the protein content and mRNA expression of HMGB1 rage in hippocampus decreased significantly (P 0.05). Compared with those before operation, the escape latency and swimming distance of group E and group R were significantly prolonged at 1 day after operation (P 0.05). Conclusion Ethyl pyruvate may improve cognitive function by down-regulating the expression of HMGB1 and rage in hippocampus.
【作者单位】：青岛市市立医院麻醉科;青岛市市立医院普外科;潍坊医学院麻醉学系临床麻醉重点实验室;
【基金】：青岛市市南区科技局立项课题(2014-14-032-YY)
【分类号】：R614

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