BMSCs和HGF在毛囊再生中的实验研究

发布时间：2018-06-17 15:53

本文选题：毛囊再生 + 骨髓间充质干细胞　；参考：《浙江大学》2015年硕士论文

【摘要】：目的：研究BMSCs和HGF在促进毛囊再生中的作用。方法：首先,制备一种胶原-壳聚糖支架,然后分别以单一负载骨髓间充质干细胞(BMSCs)的支架、单一负载肝细胞生长因子(HGF)的支架为对照组,以同时负载BMSCs和HGF的支架为实验组。我们在SD大鼠的背部皮肤处制造三个全层皮肤缺损,直径均为1.5cm,分别把以上三种支架置入大鼠的全层皮肤缺损模型中。术后,获取再生组织标本,观察大体组织学情况,制作切片进行HE染色。为了鉴定再生类毛囊结构,我们对再生组织进行了免疫组化(IHC)分析。结果：对实验组在10d、21d和35d的再生组织切片HE染色分析后发现,在10d时,见到了稀疏的团簇状的类毛囊结构；21d时,类毛囊结构渐渐增多；到35d时,毛囊结构基本成熟。IHC发现再生的类毛囊结构呈现出碱性磷酸酶(ALP)阳性,表明了再生类毛囊结构具有毛囊的特征。结果发现,同时负载BMSCs和HGF的支架能够再生出表皮与真皮组织,同时能够实现毛囊的再生。而单一负载BMSCs的支架和单一负载HGF的支架,尽管能够再生表皮和真皮组织,但不能再生出毛囊结构。结论：1.单一负载BMSCs的支架不能促进毛囊的再生。2.单一负载HGF的支架不能促进毛囊的再生。3.同时负载BMSCs和HGF的支架能够促进毛囊的再生。
[Abstract]:Objective: to study the role of BMSCs and HGF in promoting hair follicle regeneration. Methods: firstly, a kind of collagen-chitosan scaffold was prepared. Then the scaffolds loaded with bone marrow mesenchymal stem cells (BMSCs) and hepatocyte growth factor (HGF) alone were used as control group. The scaffolds loaded with both BMSCs and HGF were used as experimental group. Three full-thickness skin defects with a diameter of 1.5 cm were made in the dorsal skin of SD rats. The above three scaffolds were placed into the full-thickness skin defect model of SD rats. After operation, regenerated tissue samples were obtained, gross histology was observed and sections were made for HE staining. In order to identify the structure of regenerated hair follicles, the regenerated tissues were analyzed by immunohistochemistry (IHC). Results: after HE staining of regenerated tissue sections on the 10th day and 35th day, it was found that at the 10th day, the sparse cluster structure of the hair follicle increased gradually at the 21st day, and at the 35th day, the structure of the hair follicle increased gradually. It was found that the regenerated hair follicle structure showed alkaline phosphatase (ALP) positive, indicating that the regenerated hair follicle structure had the characteristics of hair follicle. The results showed that the scaffolds loaded with BMSCs and HGF could regenerate epidermis and dermis tissue and realize hair follicle regeneration at the same time. The scaffolds loaded with single BMSCs and HGF could regenerate the epidermis and dermis, but they could not regenerate the hair follicle structure. Conclusion 1. The scaffolds loaded with single BMSCs could not promote the regeneration of hair follicles. The scaffold loaded with HGF could not promote the regeneration of hair follicles. BMSCs and HGF loaded scaffolds can promote hair follicle regeneration.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R622

【相似文献】