硫化氢对外周动脉血管成形术再狭窄的影响及机制

发布时间：2018-06-18 07:12

本文选题：硫化氢 + 外周动脉血管成形术　；参考：《中国老年学杂志》2017年10期

【摘要】：目的研究硫化氢(H2S)对外周动脉血管成形术再狭窄的影响及机制。方法 64只清洁级新西兰白兔随机分为治疗组和对照组。建立外周血管成形术模型,治疗组每天腹腔注射125μmol/kg硫氢化钠溶液,对照组每天腹腔注射等剂量生理盐水,每组持续给药7 w。在给药期间的第3、7周,切取颈总动脉,进行HE染色,观察血管腔面积和中膜面积变化;ELISA实验检测基质金属蛋白酶(MMP)-8表达,并检测羟脯氨酸的含量变化。结果与对照组相比,治疗组血管腔面积均有显著增加(P0.01),血管中膜面积显著降低(P0.01),MMP-8含量明显降低(P0.01),羟脯氨酸含量明显降低(P0.01)。结论硫化氢通过下调MMP-8的表达,降低胶原蛋白的含量而改善外周血管成形术后出现的血管再狭窄。
[Abstract]:Objective to study the effect and mechanism of hydrogen sulfide H _ 2 S on restenosis of peripheral artery. Methods Sixty-four clean New Zealand rabbits were randomly divided into treatment group and control group. The model of peripheral angioplasty was established. In the treatment group, 125 渭 mol/kg sodium sulfide solution was injected intraperitoneally every day, and in the control group, the same dose of normal saline was injected intraperitoneally every day for 7 weeks. At the 3rd week of administration, the common carotid artery was removed for HE staining. The changes of vascular lumen area and middle membrane area were observed. The expression of MMP-8 and the content of hydroxyproline in MMP-8 were detected by Elisa. Results compared with the control group, the area of vascular lumen increased significantly in the treatment group, and the area of vascular media decreased significantly, the content of MMP-8 and the content of hydroxyproline decreased significantly (P 0.01) and P 0.01 (P 0.01) respectively. Conclusion hydrogen sulfide can improve the restenosis after peripheral angioplasty by down-regulating the expression of MMP-8 and decreasing the content of collagen.
【作者单位】：齐齐哈尔医学院基础医学院;黑龙江省出入境检验检疫局;齐齐哈尔医学院病理学院;
【基金】：齐齐哈尔医学院科研项目(YT201310);齐齐哈尔医学院院内青年基金(QY2015Q-07)
【分类号】：R654.3

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