A型肉毒素复合低剂量加巴喷丁对切口痛大鼠脊髓背角神经激肽-1受体内化的影响

发布时间：2018-06-19 20:33

本文选题：A型肉毒杆菌毒素 + 加巴喷丁　；参考：《首都医科大学学报》2017年03期

【摘要】：目的评价A型肉毒素复合低剂量加巴喷丁对切口痛大鼠行为学及脊髓背角神经激肽-1(neurokinin-1,NK-1)受体内化的影响。方法雄性SD大鼠,体质量280~300 g,6~8周龄。采用数字表法随机分为5组(n=9):对照组(Control组)、切口痛—盐水组(Saline组)、切口痛—加巴喷丁组(GBP组)、切口痛—毒素组(Bo NT/A组)、切口痛—毒素复合加巴喷丁组(G+B组)。Bo NT/A组和G+B组于造模前1 d鞘内注射Bo NT/A 0.5U,GBP组和G+B组于造模前30 min鞘内注射GBP 50 mg。切口痛模型制备后3 h,每组各随机抽取6只进行累计疼痛评分(cumulative pain scores,CPS)和机械缩足阈值(paw withdrawal threshold,PWT)的测量;每组各抽取3只,通过免疫荧光技术测定NK-1受体内化情况。结果与Control组比较,切口后3 h Saline组、GBP组、Bo NT/A组和G+B组右后足CPS升高、PWT降低、脊髓背角NK-1受体内化神经元数目上调(P0.05);与Saline组比较,Bo NT/A组和G+B组CPS降低、PWT升高、脊髓背角NK-1受体内化神经元数目下调(P0.05),GBP组差异无统计学意义(P0.05);与Bo NT/A组比较,G+B组CPS降低、PWT升高、脊髓背角NK-1受体内化神经元数目下调(P0.05)。结论单独应用低剂量GBP对大鼠切口痛无效,Bo NT/A复合低剂量GBP治疗切口痛大鼠的术后疼痛有明显的协同作用,疗效优于单独应用Bo NT/A,其镇痛机制可能与抑制切口痛大鼠脊髓背角NK-1受体内化有关。
[Abstract]:Objective to evaluate the effects of botulinum toxin A (Botulinum A) combined with low dose of gabapentin on the behavior and the internalization of neurokinin-1 (NK-1) receptor of spinal dorsal horn neurokinin-1 in rats with incision pain. Methods male Sprague-Dawley rats with body mass of 280 ~ 300 g / L and 8 weeks of age were used. Five groups were randomly divided into 5 groups by digital table: control group, saline group, incision pain plus gabapentin group GBP group, incision pain toxin group Bo NT / A group, incision pain toxin combined with Bapentin group, incision pain toxin combined with Bappentin group, G group. Bo NT / A group. Group G B and group G B were injected intrathecal injection of Bo NT / A 0.5 U GBP 1 d before model making, and group G B injected 50 mg of GBP 30 min before modeling. At 3 hours after the establishment of incision pain model, 6 rats in each group were randomly selected for measurement of cumulative pain score (pain score) and mechanical foot contraction threshold (paw withdrawal thresholdpain), and 3 rats in each group were selected for the determination of NK-1 receptor internalization by immunofluorescence technique. Results compared with the control group, the CPS of the right hind foot in the Saline group increased and the number of NK-1 receptor internalized neurons in the dorsal horn of spinal cord increased, compared with the Saline group, the CPS of the Bo NTA group and the G B group decreased the PWT increase, compared with the Saline group, the CPS of the Bo NT / A group and G B group decreased the PWT level. There was no significant difference in the number of NK-1 receptor internalized neurons in the dorsal horn of spinal cord (P 0.05), but the number of CPS in group G B was lower than that in group B, and the number of neurons internalized in NK-1 receptor in the dorsal horn of spinal cord was down-regulated (P 0.05). Conclusion low dose GBP alone has a synergistic effect on postoperative pain in rats with incision pain. The analgesic mechanism may be related to the inhibition of NK-1 receptor internalization in the spinal dorsal horn of rats with incision pain.
【作者单位】：首都医科大学附属北京朝阳医院麻醉科;
【基金】：国家自然科学基金(81571065,81428008,81400909)~~
【分类号】：R614

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