尼莫地平复合7.5%高渗盐水对七氟醚诱导老龄大鼠海马神经元凋亡的影响

发布时间：2018-06-24 02:56

本文选题：尼莫地平 + 高渗盐水　；参考：《临床麻醉学杂志》2017年12期

【摘要】：目的探讨尼莫地平复合7.5%高渗盐水对七氟醚诱导老龄大鼠海马神经元凋亡的影响。方法健康雄性Wistar大鼠96只,18月龄,体重450~500g,采用随机数字表法将其分为四组(n=24):对照组(C组)、尼莫地平组(N组)、高渗盐水组(HS组)和尼莫地平+高渗盐水组(NHS组)。C组腹腔和尾静脉注射生理盐水;N组腹腔注射尼莫地平1 mg/kg,尾静脉注射生理盐水;HS组尾静脉注射7.5%高渗盐水4 ml/kg,腹腔注射生理盐水;NHS组腹腔注射尼莫地平1mg/kg,尾静脉注射7.5%高渗盐水4ml/kg。30min后四组大鼠吸入3%七氟醚2h。于麻醉前1d、麻醉后1、3、7d行Morris水迷宫实验,实验结束后每组随机处死8只大鼠,取海马组织,采用流式细胞术测定海马神经元凋亡率和胞浆钙离子浓度;采用RT-PCR法测定海马Bcl-2、Bax的mRNA表达量。结果与C组比较,N、HS、NHS组大鼠麻醉后1、3、7d逃避潜伏期明显缩短,穿越原平台次数明显增加,麻醉后1、7d海马神经元凋亡率和胞浆钙离子浓度明显减低,Bcl-2 mRNA表达量明显上调,Bax mRNA表达量明显下调,Bax/Bcl-2比值明显降低(P0.05);与NHS组比较,N和HS组大鼠麻醉后1、3、7d逃避潜伏期明显延长,穿越原平台次数明显减少,麻醉后1、7d海马神经元凋亡率和胞浆钙离子浓度明显增高,Bcl-2 mRNA表达量明显下调,Bax mRNA表达量明显上调,Bax/Bcl-2比值明显升高(P0.05)。结论尼莫地平复合7.5%高渗盐水可抑制钙超载,降低七氟醚诱导老龄大鼠海马神经元凋亡率,且其作用优于单独给药。
[Abstract]:Aim to investigate the effect of nimodipine combined with 7.5% hypertonic saline on the apoptosis of hippocampal neurons induced by sevoflurane in aged rats. Methods 96 healthy male Wistar rats were 18 months old. The body weight was 450 ~ 500g. It was randomly divided into four groups: control group (group C), nimodipine group (group N), hypertonic saline group (HS group) and nimodipine hypertonic saline group (NHS group). C group was injected intraperitoneally and caudal intravenously with normal saline. One mg / kg nimodipine was injected intravenously, 7.5% hypertonic saline 4 ml / kg was injected into caudal vein of HS group, 1 mg / kg nimodipine was injected intraperitoneally in NHS group, and 3% sevoflurane was inhaled by caudal vein after 7.5% hypertonic saline 4ml/kg.30min. Morris water maze test was performed 1 day before anesthesia and 1 day 3 days after anesthesia. Eight rats in each group were randomly killed at the end of the experiment. The hippocampal tissue was taken out. The apoptosis rate of hippocampal neurons and the concentration of Ca ~ (2 +) in hippocampal neurons were measured by flow cytometry. The expression of Bax mRNA in hippocampus was measured by RT-PCR. Results compared with group C, the escape latency of NHS group was significantly shorter than that of group C, and the times of crossing the original platform increased significantly. The apoptotic rate of hippocampal neurons and cytoplasmic calcium concentration significantly decreased the expression of Bcl 2 mRNA on the 1st and 7th day after anesthesia, the expression of Bax mRNA decreased significantly (P0.05), and the escape latency of rats in the N and HS groups was significantly longer than that in the NHS group on the 1st and 3rd day after anesthesia, and the expression of Bax mRNA was significantly down-regulated (P0.05), and the escape latency of the rats in the N and HS groups was significantly longer than that in the NHS group. The frequency of traversing the original platform was significantly decreased, and the apoptotic rate of hippocampal neurons and the concentration of Ca ~ (2 +) in the hippocampus were significantly increased on the 1st and 7th day after anesthesia. The expression of Bax mRNA was down-regulated and the ratio of Bax / Bax mRNA was upregulated significantly (P0.05). Conclusion Nimodipine combined with 7.5% hypertonic saline can inhibit calcium overload and decrease apoptosis rate of hippocampal neurons induced by sevoflurane in aged rats.
【作者单位】：河北医科大学第三医院麻醉科;
【基金】：国家自然科学基金面上项目(81771134) 河北省政府资助临床优秀人才培养和基础课题研究项目(361005)
【分类号】：R614

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