血红素氧合酶-1在七氟醚后处理心肌缺血再灌注损伤保护中的作用

发布时间：2018-06-26 05:16

本文选题：心肌缺血再灌注损伤 + 七氟醚　；参考：《中西医结合心脑血管病杂志》2017年09期

【摘要】：目的研究七氟醚后处理对心肌缺血再灌注损伤的保护作用,探讨血红素氧合酶-1(HO-1)的发挥作用。方法健康雄性SD大鼠30只,体重230g~260g,采用随机数字标示法分为5组,每组6只。假手术组(S组)、缺血再灌注组(I/R组)、七氟醚后处理组(SP组)、七氟醚后处理加用锌原卟啉(ZnPP)组(SP+Zn组)、锌原卟啉组(Zn组)。S组丝线穿过冠状动脉前降支(LAD)不结扎,I/R组、SP组、SP+Zn组、Zn组结扎30min后松开结扎线并再灌注120min。I/R组、Zn组再灌注前3min分别单次股静脉注射生理盐水1mL,Znpp(5μg/kg)稀释液1mL并吸入纯氧3min;SP组、SP+Zn组再灌注前3min分别单次注射生理盐水1mL,ZnPP(5μg/Kg)稀释液1mL并吸入2%七氟醚3min。再灌注满120min后,(1)取抽取动脉血用酶联免疫吸附法(ELISA)测血清肌钙蛋白(cTn-I)含量。(2)取心尖缺血组织测超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。(3)用蛋白印迹法(Westernblot)测心尖缺血组织中HO-1含量。结果与S组相比,其余各组cTn-I、HO-1、MDA含量均明显升高,SOD活性明显降低,差异有统计学意义(P0.05);与I/R组比较,SP组cTn-I、MDA含量明显下降,HO-1含量、SOD活性明显升高,差异有统计学意义(P0.05);I/R组、SP+Zn组、Zn组两两比较cTn-I、HO-1、MDA含量和SOD活性,差异无统计学意义(P0.05)。结论 HO-1在七氟醚后处理对心肌缺血再灌注损伤的保护机制中起关键作用。
[Abstract]:Objective to study the protective effect of sevoflurane on myocardial ischemia reperfusion injury and to explore the role of heme oxygenase-1 (HO-1). Methods 30 healthy male Sprague-Dawley rats weighing 230 g or 260 g were randomly divided into 5 groups with 6 rats in each group. Sham operation group (S group), ischemia reperfusion group (I / R group), sevoflurane post-treatment group (SP group), sevoflurane post-treatment plus zinc protoporphyrin (ZnPP) group (SP group), zinc protoporphyrin group (Zn group). Sp / SP / Zn group after ligation of 30min and 120 minutes after reperfusion / reperfusion, 3min was injected intravenously into the femoral vein with 1 mL of normal saline (5 渭 g/kg) diluent, respectively, and before reperfusion in SP group with pure oxygen for 3 minutes. 3min was injected into 3min before reperfusion in the SP group with pure oxygen for 3 minutes. 1 mL saline 1 mL ZnPP (5 渭 g / kg) diluted solution and 2% sevoflurane inhaled for 3 min. After reperfusion with 120min, (1) the serum levels of cTn-I were measured by enzyme-linked immunosorbent assay (Elisa), (2) the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in apical ischemia tissue were measured by Western blot. (3) Western blot was used to measure the content of malondialdehyde (MDA). The content of HO-1 in blood tissue. Results compared with group S, the content of cTn-IHO-1 MDA in other groups increased significantly and the activity of SOD decreased significantly (P0.05), and the content of cTn-IO-1MDA in SP group decreased significantly compared with that of group I / R, and the activity of SOD in SP group was significantly increased as compared with that in group I / R. The difference was statistically significant (P0.05). There was no significant difference in MDA content and SOD activity between SP Zn group and SP Zn group (P0.05). Conclusion HO-1 plays a key role in the protective mechanism of sevoflurane post-treatment on myocardial ischemia-reperfusion injury.
【作者单位】：山西医科大学;山西博爱医院;山西医科大学第一附属医院;
【基金】：山西省自然科学基金面上项目(No.2014011040-9)
【分类号】：R542.2

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