外源性硫化氢对去卵巢后骨质疏松症大鼠股骨生物力学性能的影响

发布时间：2018-06-28 01:10

本文选题：原发性骨质疏松症 + 内源性H2S　；参考：《南华大学》2015年硕士论文

【摘要】：目的:通过对骨密度、生物力学指标及一些简单的宏观物理指标测量,进一步证实H2S参与骨骼代谢的调控,并探讨H2S对于骨骼生物力学性能影响,为骨质疏松的治疗提供一种新思路。方法:将48只6月龄未孕雌性SPF级近交系SD大鼠以12只为一组,随机分为4组:假手术组(仅手术探查双侧卵巢)、手术组(手术切除双侧卵巢)、低剂量Na HS注射组(切除双侧卵巢12W后再以剂量30μmol/kg/d腹腔注射Na HS 12w)、高剂量Na HS注射组(切除双侧卵巢12W后再以剂量90μmol/kg/d腹腔注射Na HS 12w),历时共24周后,对四组大鼠左侧股骨行骨密度(BMD)检测,体重股骨湿重和股骨中点直径测量,及三点弯曲力学试验。观察并分析各组大鼠在这些指标上的差异。结果:相对单纯手术组高剂量Na HS注射组的骨密度及各项目生物力学指标均有明显改善,部分参数上能与假手术组水平接近.结论:H2S确实能影响骨骼代谢,并可增加去卵巢后骨质疏松大鼠股骨的矿物沉积,改善骨质疏松大鼠股骨的生物力学性能。
[Abstract]:Objective: to investigate the effects of H2S on bone biomechanical properties by measuring bone mineral density, biomechanical indexes and some simple macroscopic physical indexes, and to further confirm that H2S is involved in the regulation of bone metabolism, and to investigate the effects of H2S on bone biomechanical properties. To provide a new idea for the treatment of osteoporosis. Methods: a total of 48 female SPF inbred SD rats aged 6 months were divided into 12 groups. They were randomly divided into four groups: sham operation group (only bilateral ovary exploration), operation group (resection of bilateral ovaries), low dose NaHS injection group (30 渭 mol/kg/d intraperitoneal injection for 12 weeks after bilateral ovarian resection), and high dose NaHS injection. After 12 weeks of ovariectomy and intraperitoneal injection of 90 渭 mol/kg/d intraperitoneal injection of Na HS for 12 weeks, the radiation group lasted for 24 weeks. Bone mineral density (BMD), wet weight and midpoint diameter of femur, and three-point bending mechanics test were measured in the left femur of the four groups. To observe and analyze the difference of these indexes in each group. Results: the bone mineral density and biomechanical indexes of each item in the high dose NaHS injection group were significantly improved compared with those in the simple operation group, and some of the parameters were similar to those in the sham operation group. ConclusionH2S can affect bone metabolism, increase mineral deposition in femur of ovariectomized rats, and improve biomechanical properties of femur of osteoporosis rats.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.3

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