脊髓损伤大鼠触液神经元中p75NTR的表达

发布时间：2018-06-28 22:41

本文选题：脊髓损伤 + 触液神经元　；参考：《中国脊柱脊髓杂志》2017年04期

【摘要】：目的 :探讨大鼠触液神经元(cerebrospinal fluid-contacting neurons,CSF-CNs)p75神经营养因子受体(p75 neurotrophin receptor,p75NTR)在脊髓损伤后的表达变化。方法 :成年雌性Sprague-Dawley(SD)大鼠36只,按随机数字表法分为正常对照组(6只)、假手术组(6只)和脊髓损伤组(24只),脊髓损伤组采用Allen′s打击模型(10g×3cm)在大鼠脊髓T10段造成急性脊髓损伤,分别于损伤3d、1周、2周、4周后进行取材;对照组不做任何处理,假手术组只暴露脊髓,不击伤脊髓。对各组大鼠运动功能行BBB评分,各时间点取材行病理切片HE染色观察。取材前48h侧脑室注射霍乱毒素B亚单位与辣根过氧化物酶复合物(CB-HRP)特异性标记触液神经元。处死大鼠后,取损伤的脊髓节段10mm,用免疫荧光双标法检测触液神经元p75NTR的表达,ImagePro Plus计数目标神经元CB-HRP/p75双阳性细胞的数目。结果 :假手术组各时间点BBB评分均为21.0±0;脊髓损伤组在术后3d、1周、2周、4周各时间点BBB评分分别为3.20±0.81、10.73±1.02、12.48±1.86、13.29±1.93,两组各时间点差异均具有统计学意义(P0.05)。HE染色可见正常对照组和假手术组脊髓组织结构完整,细胞形态正常;脊髓损伤组脊髓组织结构紊乱,神经元变性坏死,胶质细胞增生,胶质瘢痕和脊髓空洞形成。免疫荧光双标示正常对照组和假手术组可见少量CB-HRP/p75双阳性细胞,计数分别为5.16±0.55、4.31±0.61,两组比较差异无统计学意义(P0.05);脊髓损伤组伤后3d、1周、2周CB-HRP/p75双阳性细胞数分别为13.35±1.53、21.68±2.15、16.26±2.09,与正常对照组和假手术组比较差异均有统计学意义(P0.05),伤后4周时,CB-HRP/p75双阳性细胞数为4.83±0.73,与正常对照组和假手术组比较差异无统计学意义(P0.05)。结论 :p75NTR可在大鼠脊髓触液神经元中表达,且在脊髓损伤后表达增加,触液神经元可能通过p75NTR参与脊髓损伤的修复过程。
[Abstract]:Aim: to investigate the expression of p75 neurotrophin receptor p75 NTR in cerebrospinal fluid-contacting neurons (CSF-CNs) after spinal cord injury. Methods: there were 36 adult female Sprague-Dawley (SD) rats. The rats were randomly divided into normal control group (n = 6), sham operation group (n = 6) and spinal cord injury group (n = 24). Acute spinal cord injury (sci) was induced by Allen's model (10 g 脳 3cm). The samples were taken after 3 days and 2 weeks and 4 weeks after injury, while the control group did not do any treatment, and the sham operation group only exposed the spinal cord and did not hurt the spinal cord. The motor function of rats in each group was assessed by BBB, and pathological sections were stained by HE staining at each time point. Cholera toxin B subunit and horseradish peroxidase complex (CB-HRP) were injected into the lateral ventricle 48 hours before the injection. After the rats were killed, the injured spinal cord segment was taken at 10 mm. The expression of p75NTR in the neurons of contact fluid was detected by immunofluorescence double labeling method. The number of CB-HRP / p75 double positive cells in the target neurons was counted by ImagePro Plus. Results: the BBB score of sham operation group was 21.0 卤0, and that of spinal cord injury group was 3.20 卤0.81n 10.73 卤1.02nil 12.48 卤1.86n 13.29 卤1.93 at each time point, and the BBB score of spinal cord injury group was 3.20 卤0.81n 10.73 卤1.02n 12.48 卤1.86n 13.29 卤1.93, respectively. There were significant differences between the two groups at each time point (P0.05) .HE staining showed that the normal control group and HE staining group had statistical significance (P0.05). The spinal cord of sham-operated group was intact, In the spinal cord injury group, the structure of the spinal cord was disordered, the neurons degeneration and necrosis, the proliferation of glial cells, the formation of glial scar and syringomyelia. Immunofluorescence double labeling showed a small number of CB-HRP / p75 double positive cells in normal control group and sham operation group. The number of CB-HRP / p75 double positive cells in spinal cord injury group was 13.35 卤1.53 卤21.68 卤2.15n 16.26 卤2.09, which was significantly higher than that in normal control group and sham operation group (P0.05), and CB-HRP / p75 double positive cells in spinal cord injury group were significantly higher than those in normal control group and sham operation group at 4 weeks after injury (P0.05), and the number of CB-HRP / p75 double positive cells in spinal cord injury group was 13.35 卤1.53n 21.68 卤2.15n 16.26 卤2.09 respectively (P0.05). The number of double positive cells was 4.83 卤0.73, which was not significantly different from that of normal control group and sham operation group (P0.05). Conclusion the expression of p75NTR in rat spinal cord contact neurons is increased after spinal cord injury, and the fluid contact neurons may participate in the repair of spinal cord injury through p75NTR.
【作者单位】：贵州医科大学;贵州医科大学附属医院急诊骨科;
【基金】：贵州省科技计划(黔科合LH字[2014]7149)
【分类号】：R651.2

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